荷斯坦牛乳房炎相关基因多态性及其与体细胞评分的相关性研究-动物遗传育种与繁殖专业论文.docxVIP

of isoleucine→valine.AA(0.308), AB(0.490) and BB(0.202) genotypes were found at T853A and G855A sites in Holstein cows using PCR-SSCP and in Hardy-Weinberg equilibrium. LSM of SCS for BB genotype was significantly lower than that for AB (P0.01) or AA (P0.0001), LSM of SCS for AB genotype was significantly lower than that for AA (P0.01). CC (0.332) and CD(0.668) genotypes were detected at G1793A site using PCR-RFLP with BsiYⅠ,EE(0.178) and EF(0.822) genotypes at C1859A site with BstXⅠ,GG (0.356) and GH(0.644) genotypes at G1934A site with BsiYⅠ, and II(0.260), IJ(0.567) and JJ(0.173) genotypes at G1934A site with BclⅠ.One of four sites, only at A1980G, was in Hardy-Weinberg equilibrium LSM of SCS for JJ genotype was significantly lower than that for IJ (p0.01) or II (p0.0001),LSM of SCS for IJ genotype was significantly lower than that for II (p0.01). The results implied that both B and J alleles of TLR6 gene are two potential DNA markers for improving mastitis resistance in dairy cattle. Key Words: Holstein cattle，somatic cell score，BoLA-DRB3 gene，Toll like receptor， mastitis，SNP 略词语表（Abbreviation） 英文缩写 英文全称 中文名称 MHC Major histocompatibility complex 主要组织相容性复合物 BoLA Bovine lymphocyte antigen 牛淋巴细胞抗原 TLR Toll like receptor Toll 样受体 SCC Somatic cell count 体细胞数 SCS Somatic cell score 体细胞评分 DNA Deoxyribonucleic acid 脱氧核糖核苷酸 He Heterozygosity 杂合度 Het Heterocluplex analysis 杂交双链分析 Ho Homozygosity 遗传纯合度 MAS Maker assisted select 标记辅助选择 PAGE Polyacrylamide gel electrophoresis 聚丙烯酰胺凝胶电泳 dNTP Deoxynucleotide triphosphate 三磷酸脱氧核糖核苷 EB Ethidium bromide 溴化乙啶 EDTA Ethylene diaminetetraacetic acid 乙二胺四乙酸 Amp Ampicilin 氨卞青霉素 Ne Effective number of alleles 有效等位基因数 PCR Polymerase chain reaction 聚合酶链式反应 RFLP Restriction fragmengts length polymorphism 限制性片段长度多态性 SNP Single nucleotide polymorphism 单核苷酸多态性 SSCP Single-strand conformation polymorphism 单链构象的多态性分析 论文独创性声明 本人郑重声明：所呈交的学位论文是我个人在导师指导下进行研究工作所取得的 成果。尽我所知，除了文中特别加以标注和致谢的地方外，学位论文中不包含其他个 人或集体已经发表或撰写过的研究成果，也不包含为获得安徽农业大学或其它