Ghrelin在情感枢与胃功能调控作用研究-病理生理学专业毕业论文.docx

AbstractObjective：The Abstract Objective：The aim of the present study was(1)To investigate the effects of ghrelin in hippocarnpal CAl area on gastric motility and its mechanism by which hypothalamic ghrelin is involved in the regulation of gastric motility；(2)To study the influence of ghrelin 011 the calcium—activated K+current of guinea pig gastric myocytes，and the function of motilin with the regulation of NO；(3)To explore the effects and mechanisms of Ghrelin on the learning and memory in rats． Methods：(1)measale the gastric motility in freely moving rats to eXalTl the effects and the mechanism of microinjection ghrelin into hippocampal CA 1 alea on gastric movement．(2)Patch clamp experiment：Acute dissociation of guinea pig gastric myocytes and observe the influence and mechanism of ghrelin on calcium·activated K+current of gastric myocytes using whole—cell patch-clamp techniques．(3) Step-down test and morris water maze were used to test the effects of learning and memory ability by means of microinjecting ghrelin into hippocampal CA 1 alea in rats． Results：(1)The amplitude ofgastric motility Was decreased by miroinjection of the Ghrelin into hippocampus CA 1 Rlea(P0．o D．The effect was increased by L-NAME(P0．05)and was decreased by L—AA口o．05)．(2)The results of path-clamp：Ghrelin can inhibit the calcium—activated K+current of guinea pig gastric myocytes if,o．05)．(3)Ghrelin Call improve the learning and memory ability of rats 伊O．05)． Conclusion：Ohrelin Call decrease the amplitude of gastric motility,inhibit the calcium．activated K+current of guinea pig gastric myocytes and improve the learning and memory ability of rats．And all of the functions are implemented by No pathway． Postgraduate student：Xiao—fang Wei(pathophysiology) Directed by：Prof．Luo Xu Key words：Ghrelin；Hippocampus；Gastric motility；Learning and memory； NOS 学位论文独创性声明学位论文独创性声明 学位论文独创性声明 学位论文独创性声明 本人声明，所呈交的学位论文系本人在导师指导下独立完成的研究成果。文中 依法引用他人的成果，均已做出明确标注或得到许可。论文内容未包含法律意义上 已属于他人的任何形式的研究成果，也不包含本人已用于其他学位申请的