Ghrelin在雄性食蟹猴消化系统与生殖系统中的免疫组化定位研究-基础兽医学专业业论文.docx

细胞主要分布在小肠腺，少量分布于肠绒毛上皮中。根据细胞顶端是 细胞主要分布在小肠腺，少量分布于肠绒毛上皮中。根据细胞顶端是 否到达腺体腔面，可将胃肠道中的Ghrelin细胞分为“开放型和“封 闭型”。胰腺的外分泌部和胰岛中均有阳性细胞分布。 5．Ghrelin阳性细胞在组织中多呈散在分布。细胞大小不一、形 态各异，多呈圆形、卵圆形、锥体形、长柱形及其他不规则形。胃中 主要为封闭型细胞，十二指肠中存在封闭型和开放型两种类型，空肠 中则主要为开放型细胞。 6．Ghrelin免疫阳性细胞被染为棕色到棕黑色，细胞界限不清晰， 在有些细胞的中央或边缘位置可见半透亮的细胞核，说明Ghrelin阳 性物质分布在细胞质中，且阳性产物在胞质中的分布不均 关键词：Ghrelin 食蟹猴消化系统生殖系统免疫组化定位 万方数据 STUDY STUDY oN IMMUNoHISTOCHEMICAL LoCALIZATIoN OF GHRELIN IN THE DISGETIVE SYSTEM AND REPRoDUCTIVE SYSTEM oF MALE MACACA FASCICULARIS ABSTRACT The study explored the localizition of Ghrelin in the digestive and reproductive systems of the Macaca fascicularis by immunohistochemical． The research that studies the distribution，content and cell morphology of Ghrelin positive cells in the two systems lay the foundation of morphology basis for the functional study of Ghrelin in Macaca fascicularis．The major resuts were as follows： 1．Immunohistochemical staining shows that Ghrelin positive cells distributes both in the digestive system and reproductive system of Macaca fascicularis． 2．In digestive system，Ghrelin positive cells mainly distributes in the stomach，duodenum，the front-j ejunum and mid-jejunum，as well as pancreas，but in the oral mucosa，tongue，esophagus，back-jejunum， ileum，colon，cecum，rectum and liver were no distribution．In reproductive system，Ghrelin positive cells mainlty distributes in the testis， epidydimis and sperma ducts． 3．In stomach，the concentration of Ghrelin positive cells is highest 万方数据 and and mainly distributes in gastric glandular region．The number of positive cells in the cardiac gland and pyloric gland were also higher．The concentration of intestinal was lower than stomach，and the concentration gradually reduced from duodenum to mid-jejunum．The number of Ghrelin positive cells in pancreas were close to jejunum． 4．Ghrelin cells were mainly distributed in the gastric body and bottom of the glands，the distribution in the neck of glands and gastric epithelial was minimal．Ghrelin cells were main