miR168a沉默转基因低实率水稻突变体的基因组学分析-植物学专业毕业论文.docx

他SVs类型(长片段插入LI、短段插入SI、缺失DEL及倒位INV) 他SVs类型(长片段插入LI、短段插入SI、缺失DEL及倒位INV) 均比野生型多，③19．9．4特有的SVs总数是99，037个，多于野生型 35，934个。此外，比对野生型的全基因组序列，⑧19．9—4中有106个 拷贝数变异(CNVs)，其中在第4号和12号染色体上CNVs均为 1 6个，第3号染色体上只有2个。 4．依据WGR分析和PCR分析验证，初步确定③19．9．4中外源 T-DNA(GCS．IPSl．MIMl68a)在Kasalath染色体上有2个可能的插入 位点，其一在3号染色体2609601bp之后；其二是5号染色体的 604415bp至604502bp之间。生物信息学分析表明，第一个插入位点 可能涉及2个基因，其一是LOC—Os0395334，功能预测为潜在的编 码蛋白质的基因，但具体的生物学功能还未见报道；另一个是 OS03G0146800(非编码RNA基因)。这两个基因是否与种子结实率 调控相关，还有待进一步研究。第二个插入位点属于基因间隔区，可 能不影响基因功能。 总之，③19—9—4与野生型的全基因组重测序(WGR)比对分析 结果表明，外源T-DNA导入野生型基因组后，引起了受体基因组 SNPs、InDels、SV等结构和CNVs的明显差异变化，并因此导致了 基因表达和氨基酸组分的变化。这些结果为深入探讨种子结实性状的 分子机制提供了重要线索。 关键词：全基因组重测序，SNP，InDels，水稻结实率 III 万方数据 N 万方数据 AbstractRice Abstract Rice is one of the most important crops in the world，and it is also a model plant in the study of functional genomics of monocotyledon cereals．The seed setting rate is one of the important factors markedly affecting rice yield and has been focused in rice genetic breeding and production．After transducing T-DNA(MIM 1 64c，OE 1 64c， MIM 1 68a，OE 1 68a)into kasalath，a large of transgenic mutants were achieved．Through the successive selection and identification of biological characteristics of these transgenic lines from T 1 to T4 generation，a transgenic line@1 9—9—4 was screened out，whose seed setting rate is significantly lower than wild type．In this study,we attempt to provide the theoretical bases to explore the molecular mechanism associated with seed setting rate of rice，the fertility and viability of pollens of③1 9—9—4 was measured and the alignment of whole genome resequencing(WGR)of this mutant line was compared with the wild type． The main results are as follows： 1．From T 1 to T4 generation lines created by transforming exogenous T-DNA(MIM 1 64c，OE 1 64c，MIM 1 68a，OE 1 68a)into conventional rice kasalath，many phenotypes of mutation were acquired，including plant height，tiller numbeg leaf shape，heading date，grain shape，effective panicle number,1 000 grain weight，seed storage t