Mir4835p在食管鳞癌清和组织中的表达及其调控机理的初步探究.docx

ABSTRACTEsophageal ABSTRACT Esophageal squamous cell carcinoma(ESCC)is one ofthe most common types ofmalignacies in China．Most ESCC patients diagnosed at middle to late stages with poor prognosis due to the lack of effective method for early diagnosis．Therefore，it is needed to find effective method for early diagnosis． MiRNAs arc a group of endogenous small non-coding RNAs that regulate ESCC development and progression by repressing their expression of specific target genes．In this stlldy,firstly,the four miRNAs (miR-25-3p，miR-223—3p，miR-373—3 and miR-483·5p)wgre selected according to serum miRNAs microarray in some reports；Secondly,the expression levels of four miRNAs and the mRNA related to miR．-483·5p were measured by RT-qPCR，and the methylation levels of related genes detected by MSP method．The study was aimed to explore the expression levels of four miRNAs in ESCC and the molecular regulation mechanism ofmiR-483—5p．The results wfffe follows： 1．The expression levels of miR-25-3p，miR-223-3p，miR-373—3p，miR-483—5p were significantly up-regulated in patients’seruln compared with those in healthy volunteers p0．0 1)．ROC curve analyses revealed that the AUC value for miR-25·3p，miR-223-3p，miR,373-3p and miR-483·5p was O．794(95％C1 0．659-0．928；sensitivity=81．0％；specificity=66．7％)，0．839(95％CI：0．717-0．961；sensitivity=100．0％； specificity=61。9％)，O．873(95％CI：0．763-0．983；sensitivity=90．5％；specificity=81．O％)，and 0．927(95％ CI：0．850-0．1．000；sensitivity=95．2％；specificity=8 1．O％)，respectively．In addition，the expression level of miR-483-5p was correlated with the TNM stage pD．叻and lymph node metastasia(萨O．05)．As result， four sen珊miRNAs were observed to be significantly reduced after surgical removal of the tunlors 够(O．oo．We have demonstrated that four serum miRNAs which had high sensitivity and specificity Can be IlI 万方数据 used used for the early prediction of ESCC．PCR products amplified using stem·loop primers from selqlm and tissue of ESCC patients were right by sequencing．So the