IGFBp1参与老龄促进动脉粥样化的作用和机制.docx

第三军医大学硕士学位论文PBS 第三军医大学硕士学位论文 PBS phosphate buffer 磷酸盐缓冲液 PMSF phenylmethanesulfonyl fluoride 苯甲基磺酰氟 PVDF polyvinylidene difluoride 聚偏二氟乙烯 P5 passage 5 第5代 P20 passage 20 第20代 RNase ribonuclease RNA酶 ROC receiver operating characteristic curve 受试者工作特征曲线 SA—p-gal senescence associate-p-galactosidase 衰老相关．p一半乳糖苷酶 SBP systolic blood pressure 收缩压 siRNA small interfering RNA 小干扰RNA TC total cholesterol 总胆固醇 TG triglyceride 甘油三脂 TNF tumor necrosis fairor 肿瘤坏死因子 WHR waist-hip ratio 腰臀比 2 万方数据 第三军医大学硕士学位论文The 第三军医大学硕士学位论文 The role and mechanism of Insulin--like growth factor binding protein I in the development of aging related atherosclerosis Abstract Background： Coronary heart disease is the leading cause of morbidity and mortality in the world． The main pathological characteristic of coronary heart disease is coronary atherosclerosis． Aging is an independent risk factor of atherosclerosis．Age related vascular remodeling which attributes to mediate vascular cells’biological function facilitates the progress of atherosclerosis．Increasing age participant in the initiation and development of atherosclerosis by accelerating endothelial cell senescence and apoptosis． Several signaling pathways are involved in age—related vascular remodeling． Investigations have revealed a potential link between Notch signaling and the abnormity of age。related tissue repair,thus the growth and development associated Notch signaling may play a certain role in aging．We have made a preliminary research to make sure the role of Notch signaling in regulating the proliferation and senescence of vascular cells．In the study we found that the expression of j agged l，one kind of Notch ligands was significantly reduced in senescent endothelial cell，meanwhile the downregulation ofjaggedl contribute to the decreased secretion of Insulin-like growth factor-binding protein I(IGFBP．1、． IGFBP_1 is a protein produced mainly in the liver,and it belongs to insu