IMSPCR检测食品中的单增李特菌.docx

Abstract一●————————————————————————————————————————————————————一 Abstract 一●————————————————————————————————————————————————————一 Abstract Listeria monocytogenes is a common bacterium in nature，which can occurs both m h啪ans aIld iIl aIlimals．Listeriosis is a severe disease associated with a high case fatality rate． So if foods contain Listeria monocytogenes，it is very important to adopt scientific methods to detect tllem．IMS．PCR method cail quickly detect Listeria monocytogenes in foods· IMS utilize the principle of antigen—antibody response and do Listeria monocytogenes experiments of specificity and sensitivity．PCR is that one set of primers Was designed according to the sequences of the lap gene(P60 protein coding)of Literia monocytogenes and conducted PCR；and do Listeria monocytogenesexperiments of specificity and sensitivity· According to analyze and compare the two methods，we set up IMS—PCR and employ the fonner priIIler to do Listeria monocytogenesexperiments of specificity and sensitivity．Also Guobiao(standard method of inspection)and RealTime PeR(kit)take as the control of IMS．PCR,then analyze and compare IMS-PCR S results． IMS can quickly entrap bacteria to increase them in culture fluid(37C 14--18h)，and hemolytic reaction and rhamnose reaction can confirm if it is Literia monocytogenes，and dem趾ds 3-7 days；sensitivity can attain 107．PCR：Obtaining Literia monocytogenes from foods to carry out enrichment culture which need 3-5 days．Extract bacterial genome DNA to carry out PCR and deserve 230bp,which arrive at results for 4 hours，its sensitivity call attain 1 05．IMS—PCR：From fast collecting bacteria to progress enrichment culture，then deselwe 230bp after PCR,it Can detect Lisiteria monocytogenes in a day．It lessened the time of inspection，at the same time it raised detective specificity and sensitivity of Lisiteria monocytogenes· The results of IMS．PCR agree with Guobiao and Real Time PeR(kit)，then it is more convenient．faster and sensitive than Guobiao；and l