let7影响内皮细胞功能的网络调控分析.docx

山东大学硕士学位论文4．2．1 山东大学硕士学位论文 4．2．1 lncRNA比对 ．37 4．2．2 inhibitor组结果 ．．39 4．2．3 mimiC组结果 42 4．3共表达分析 ．45 4．3．1 mRNA DAVID功能富集结果 ．45 4．3．2 mRNA pathway分析结果 48 4．3．3 IncRNA与mRNA的共表达 ．．50 4．4 let一7e结合位点 57 4．5 let一7e—mRNA—IncRNA的调控网络 58 4．5．1有let一7e的结合位点的mRNA ．．58 4．5．2 let一7e对内皮细胞的调控作用 59 4．6本章小结 ．．62 第5章总结与展望 ．．63 参考文献 ．．65 致谢 69 万方数据 山东大学硕士学位论文CONTENTS 山东大学硕士学位论文 CONTENTS ABSTRACT(in Chinese) ．I ABSTRACT ．．III Chapterl Introduction ．1 1．1 Gene Microarray ．．1 1．1．1 Theprinciple ofGene chip l 1．1．2 The application ofgene chip ．3 1．1．3 The development ofgene chip ．4 1．2 miRNA and IBeRNA ．．5 1．3 let-7e 6 1．4 Clustering analysis ．．6 1．5 microRNA target prediction 7 1．6 Chapter summary ．8 Chapter2 gene chip experiment ．．9 2．1 Culture endothelial cells、ransfection and extract total RNA ．9 2．1．1．Experimental materials and equipment 。9 2．1．2 The cultivation ofhuman umbilicaI vein endothelial cells 9 2．1．3 The trailsfection of Endothelial eell 1l 2．1．4 Total RNA extraction ．12 2．2 lneRNA chip l 3 2．2．1 lncRNA genomic chip 13 2．2．2 Data source oflncRNA genome chip ．13 2．2．3 Principle ofAgilent chip ．．14 2．2．4 Experimental procedure 15 2．3 The results ofGene chip experiments ． ．．16 2．4 Chapter summary．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．．． 17 Chapter 3 The method ofmicroarray data analysis l 8 ：；．1 Flow chart ofdata analysis ． ．18 3．2 The chip data standardization ．19 3．2．1 Correction of background l9 3．2．2 Quantile standardization ．．20 3．3 Significantly differentially expressed genes 20 3．3．1 Method ofmultiplicity 一21 3．3．2 T test contorl False positive 21 3．4 Mrna．1ncRNA CO．expression 23 3．4．1 Clustering analysis． ．．． ． ． ． ． ． ． ．．． ．．．：． ． ． ． ． ． ． ．．23 3．4．2 Co．expression analysis ． ．：16 3．5 let．7e micro砌、IA target prediction ．28 3．6 Building lncRNA．1et．7e．mRNA Interaction network 28 3．7 Chapter summary．．．． ．． ． ． ． ． ． ． ． ． ． ． ．．． ． ． ． ． ． ．．． 29 万方数据 山东大学硕士学位论文Chapter4 山东大学硕士学位论文 Chapter4 The results ofGene chip dataan