miRNa122小分子调剂的合成与抗肝癌细胞作用研究.docx

北京化工大学硕士学位论文此，我们进一步确立了miRNA一122表达情况与肝癌进展和抑制之间的 北京化工大学硕士学位论文 此，我们进一步确立了miRNA一122表达情况与肝癌进展和抑制之间的 关系，为后续的治疗方案和研发出更高效的化合物药物奠定基础。 关键词：肝癌，丙肝病毒，miRNA．122，MTT法，抗癌药物 万方数据 AbstractThe Abstract The synthesis of miRNA-122 small molecule modulators and study of anti·-hepatoma cell function ABSTRACT Objective：Hepatocellular carcinoma(HCC)is one of leading causes of death in the world．Short survival time and poor prognosis of patients with hepatocellular carcinoma．Therefore，early prevention and treatment of liver cancer is particularly important．Hepatitis C virus is a maj or cause of cirrhosis and liver cancer．Early intervention is very effective and important．miRNA一1 22 is a specific microRNA in liver cancer，which Can be highly expressed and stable in normal liver cells．High expression of miRNA-1 22 can inhibite 1iver cancer cell’S invasion and metastasis，but also protect the genetic material of the hepatitis C virus． Purpose：This study aims to design and synthesize some new compounds．We hope that these compounds are capable of liver cancer and hepatitis viruses play a therapeutic role． Experimental Design and Methods：In this experiment，a total of 1 2 compounds were synthesized，including 1 1 new derivatives．Measured two tumor cell lines(HuH-7 cell lines and HepG2 cell lines)to the drug sensitivity and inhibition rate by MTT assay． ResultsConclusions：There are two compounds(Compound 7， III 万方数据 北京化工大学硕士学位论文Compound 北京化工大学硕士学位论文 Compound 8、in MiRNA-1 22 high expression HuH·7 hepatoma cell lines exhibited growth inhibition of tumor cell．One compound(Compound 9) has played a role in promoting tumor cell growth，and they are significantly different(P0．05 or PO．01)．In contrast，MiRNA一122 low expression in HepG2 cells was not observed a significant difference between the phenomenon of inhibition or promotion．Accordingly，we can depending on the experimental results and the functions and distribution of MiRNA·1 22，By establishing the development of individualized treatment programs，the appl