HBH7树脂血液灌流吸附犬内毒素血症的验分析.docx

天津医科大学硕士学位论文中文摘要 天津医科大学硕士学位论文 中文摘要 目的：探讨应用HB．H．7树脂行血液灌流吸附治疗犬内毒素血症的有效性和 安全性。 方法：将12只成年杂种犬随机分为治疗组与对照组，每组6只，内毒素血症 犬动物模型采用经中心静脉微量输液泵匀速输注内毒素0．5mg／kg，HB．H．7树脂 经过工程制备和高压灭菌处理，灌流方式采用全血灌流，连续灌流2h。分别于 灌流开始前、灌流开始后30分钟、60分钟和120分钟采集全血标本测定血浆内毒 素、血浆蛋白、电解质和白细胞、红细胞及血小板。 结果：血液灌流吸附前后比较，治疗组血浆内毒素水平明显下降(PO．05)， 对照组无明显下降(尸O．05)I两组比较差异有显著性(尸0．05)。HB．H．7树 脂对血浆蛋白有一定影响，治疗组灌流吸附结束时与灌流吸附前比较，血浆总 蛋白下降25．9％，白蛋白下降18．2％，球蛋白下降34．2％，统计学差异有显著性(P O．05)。HB．H．7树脂对白细胞、红细胞、血小板及电解质没有明显影响(P 0．05)。治疗组犬实验后均长期健康存活，对照组犬于实验结束后14--22h全部 死亡。 结论：HB．H．7树脂对内毒素吸附率高，HB．H．7树脂血液灌流治疗犬内毒 素血症安全有效。 关键词：HB．H．7树脂血液灌流内毒素内毒素血症 天津医科大学硕士学位论文Abstract 天津医科大学硕士学位论文 Abstract OBJECTIVE：Discuss the effectiveness and safety of treating the endotoxemia dog model with hemoperfusion application of liB-H一7 resin． METHODS：12 dogs were divided randomly into treatment and control groups， 6 dogs in each group．Endotoxemia of model dogs WaS performed by infusing endotoxin 0．5 megkg through central venous using micro—infusion pump．HB-H-7 resin，which WaS prepared by projective preparation and high—pressure sterilization， perfused for 2h through whole blood hemoperfusion． RESULT：The plaSma endotoxin levels of treatment group were significantly decreaSed(脚．05)after hemoperfusion,otherwise，there was no significant decline in the control group(伶O．05)，SO there were significant difference(P0．05)between the two groups．Plasma protein Was affected by HB-H-7 resin in SOme degree．Total plaSma protein declined 25．9％after perfusion,in which plasma albumin fell 1 8．2％， and globulin 34．2％，there was significant difference(P0．05)in statistics．There Was no apparent impact on the red blood cells，platelets and electrolyte．All of the model dogs in treatment group survived healthy for a long time after experiment，but all of ‘that in the control group were died after 1 4～22 h． CONCLUSION：Endotoxin Can be highly absorpted by HB·-H·—7 resin,SO it is safe and effective to treat endotoxernia dogs animal model、^，im HB-H一7 resin through hemoperfusion． Keywords：HB-H-7 resin Hemop