HIF1α VEGF在不明原因反复自然流产患者绒毛织中的表达及与MVD的关系.docx

兰州大学硕士学位论文原因之一。 兰州大学硕士学位论文 原因之一。 4)HIF一1仅、VEGF与MVD的相关关系的研究，有望为URSA的预防和治疗提供有力 的理论依据。 关键词：不明原因反复自然流产缺氧诱导因子．1a血管内皮生长因子微血管密度 II 兰州大学硕士学位论文ABSTRACT 兰州大学硕士学位论文 ABSTRACT Objecive： To examine the expression of HIF一1 aand VEGF in villus of patients with unexplained recurrent spontaneous abortion(URSA)，and to study its relation with MVD．Then to detect the effect of hypoxia and HIF—l aon the expression of VEGF in the conditional cultured cyto仃ophoblasts would provide guidance for the study of the pathogenesis of URSA． Method： Immunohistochemical SP method was used to examine the expression of HIF—l a，ⅦGF and MVD in villus of 28 URSA patients and 20 heaithy pregnant women．The result Was analyzed by SPSS l 1．5． Result： 1)Villi edema cytotrophoblast cell hyperplasia and syncytiotrophoblast cell decrease were observed in the case group．Cytotrophoblast edge sharpness，cellular nucles vascuolar,syncytiotrophoblast comparatively large to confluence one another were observed in the control group． 2)HIF-laand VEGF were．observed chiefly in plasma and membrance of． cytotrophoblasts cell and vascular endothelial cell botll in the two groups．And the mesenchymal has little stain．However the expression degree of HIF-1 aand VEGF in the villi of the case group were decreased significantly than that of the control group． 3)Angiodysplasia,lumina narrow or no apparente lumina,little blood cell in the lumina of the case group，the verage of MVD Was significant decreased in the case group than that of the control group． 4)Positive correlation Was found between HIF一1 aand VEGE Positive correlation Was found between HIF．1 0【、VEGF andⅣⅣD． Conclusion： 1)Compared to the villi of normal pregnant，there were recessive changs in the villi of the URSA． 2)HIF—l aand VEGF expression of the villus may play an important role in the normal pregnant，possibly through affecting the cytotrophoblastic invasion and placental vascular reconstruction via the modulation ofVEGF gene transcription． 3)The expression levels of