三氯异氰尿酸对斑马鱼毒性靶器官及效应分子的筛选-流行病与卫生感统计学专业毕业论文.docx

结果：1．我们研究结果发现，斑马鱼对不同浓度三氯异氰尿酸的急性 毒性反应不同。三氯异氰尿酸对斑马鱼的96h半数致死浓度为 551．056鹇／L。2．三氯异氰尿酸对斑马鱼的脑、精巢、卵巢的毒性作用 不明显。3．三氯异氰尿酸对斑马鱼肝脏造成急性损伤，损伤随着染毒 浓度的增高而增大的趋势。初步筛选出肝脏是三氯异氰尿酸毒性作用 的主要靶器官之一。4．三氯异氰尿酸暴露引起斑马鱼肝脏组织发生细 胞凋亡、组织充血、组织形成部分空洞等病理变化，细胞核和线粒体 病变最严重，可能引起斑马鱼发生疾病和死亡，并且随着染毒浓度增 加，损伤也逐渐增大。5．经过双向荧光差异电泳技术和质谱鉴定，我 们发现了BHMT、HPDA、FABPIOA、ALDOB等表达差异蛋白。6． 三氯异氰尿酸会造成斑马鱼肝脏组织细胞中的BHMT蛋白的表达抑 制。 结论：1．三氯异氰尿酸对斑马鱼急性毒性根据《全球化学品分类和标 签协调手册??(The Globally Harmonized System of Classification Labelling of Chemicals，GSH)以LCso为标准分类，属于高毒物质。2． 经三氯异氰尿酸暴露后，斑马鱼脑、精巢和卵巢病理变化不明显，肝 脏可能是三氯异氰尿酸对斑马鱼急性毒性的主要靶器官之一。 3．BHMT蛋白可能是三氯异氰尿酸对斑马鱼急性毒性的早期效应分 子。 关键词：三氯异氰尿酸，流行病学特征，急性毒性，靶器官 万方数据  ABSTRACT Objective：To elucidate the TCCA—induced acute toxicity in zebrafish this study systematically analyzed the epidemiologic feature of TCCA at different aspects(including populations，animals，organs，cells and bio—moleculars)．We identifed potential target organs and early stage biomarkers in TCCA-indued acute toxicity．The findings provided scientific basis for the risk accessment of TCCA exposure and explored the molecular mechanisms of TCCA-induced acute toxicity． Methods：ne study expounded epidemiological survey of TCCA， chlorinated disinfection by-products，and its associated disease．Provided epidemiological evidence for the experiment．Exposured zebrafish witIl different concentrations of TCCA solution，observed the acute-toxicity effect，confirmed the 96h median lethal concentration(LCso)of TCCA on zebrafish．Doses to be used were determined according to LCso．7 days after exposure， several organs were dissected and analyzed by histological assays，including brain，liver，testis and ovary tissues． Pathological changes of organs observed respectively，preliminary screened the acute—toxicity target organs of TCCA on zebrafish． Morphological changes of target organ liver cells were observed by histopathologic slide and transmission electron microscope．Effector molecules were analyzed by two-dimensional fluorescence difference gel electrophoresis(2D—DIGE