中药单体复方防治支气管哮喘的实验研究-中国科技论文在线.PDFVIP

中国中西医结合杂志2004年8月第24卷第8期CJITWM，August 2004，Vo1．24，No．8 7l7 · 中药单体复方防治支气管哮喘的实验研究 董竞成 倪 健 宫兆华 摘要 目的 观察中药单体复方(由银杏内酯、黄芩甙、川芎嗪组成)对哮喘气道变应性炎症和气道高反 应性的影响。方法 将哮喘模型豚鼠随机分为3组：模型组、中药单体复方组和色甘酸钠组。模型组：致敏 豚鼠，生理盐水雾化吸入；色甘酸钠组：致敏豚鼠，色甘酸钠生理盐水溶液4mg／(kg·d)雾化吸入；单体复方 组：致敏豚鼠，银杏内酯2mg／(kg·d)加黄芩甙7．5mg／(kg·d)加川芎嗪3．75mg／(kg·d)雾化吸入。自造模第 l天起雾化吸入用药，疗程均为 l4天。检测并比较各组豚鼠血嗜酸细胞计数和嗜酸细胞阳离子蛋白 (ECP)、支气管肺泡灌洗液(BALF)细胞总数、嗜酸细胞计数和ECP；比较组织胺对气道反应性的影响和治疗 结束后的病理改变。结果 中药单体复方组对血和支气管肺泡灌洗液嗜酸细胞计数和支气管肺泡灌洗液细 胞总数均有明显减少，与模型组比较，差异有显著性(P0．05，P0．01)；ECP各组比较，差异无显著性；气 道反应性检测显示中药单体复方组对气道高反应性有明显的抑制。应用中药单体复方组的豚鼠病理气道炎 症性改变较模型组明显减轻。结论 由银杏内酯、川芎嗪、黄芩甙等中药有效成分组成的复方吸入剂．有抗 哮喘气道变应性炎症和气道高反应性作用。提示中药单体复方可以从多角度、多靶点抑制具有多种发病环 节的哮喘炎症，从而有效地治疗哮喘。 关键词 中药单体复方；支气管哮喘；气道炎症：抗炎治疗 Experimental Study on Prevention and Treatment of Bronchial Asthma by Compound Chinese Herbal Monomer Recipe DONG Jing—cheng，NI Jian，GONG Zhao—hua Institute ’Pulmonary and Atopic Diseases by Integra— tire Ch inese and Western Medicine，Huashan Hospital Affiliated to Fudan University，Shanghai(200040) objective To observe the effect of compound Chinese herbal monomer(CHM)recipe，consisted of ligus— trazin(3．75 mg／kg’d)，baicalin(7．5 mg／kg。d)and ginkgolide(2 mg／kg·d)，on airway atopic inflammation and hyper—responsiveness in asthma，Methods Model guinea pigs of asthma were randomly divided into three groups，the model group，the CHM group and the cromlyn sodium(CS)group，they were treated by atomizing inhalation with normal saline，CHM and CS respectively．The eosinophil count and eosinophil cation protein (ECP)in blood and bronchial alveolar lavage fluid(BALF)，and total cell count in BALF were measured and compared， And the effect of treatment on the airway hyperresponsiveness and pathology among groups were compared，Results CHM showed significant