粗毛栓菌（Trameteshirsuta）D2固态发酵山核桃.PDF

微生物学通报 Oct. 20, 2014, 41(10): 1955−1960 Microbiology China © 2014 by Institute of Microbiology, CAS tongbao@ DOI: 10.13344/j.microbiol.china.130920 研究报告 粗毛栓菌(Trametes hirsuta) D2 固态发酵山核桃 蒲壳产漆酶的营养条件研究 * 杨柳 吕丹丹 邓杰勇 曹焕英 叶明 ( 230009) 摘 要【目的】为提高漆酶产量，降低生产成本，以山核桃蒲壳作为基质，对粗毛栓菌D2 固态发酵产漆酶的营养条件进行研究。【方法】对不同碳源、氮源、碳氮比、蒲壳含量对漆酶 产量的影响进行分析。【结果】山核桃蒲壳是粗毛栓菌生长的良好载体，能够促进漆酶的合成。 粗毛栓菌D2 漆酶固态发酵培养基干物质组成为：山核桃蒲壳40% (质量比) ，玉米粉24% (质 量比) ，菜籽饼粉36% (质量比) 。发酵6 d 时，漆酶活性为126.8 U/g 干基。【结论】粗毛栓菌固 态发酵山核桃蒲壳产漆酶具有效率高，生产成本低的优点，具有潜在的工业化应用前景。 关键词粗毛栓菌，固态发酵，漆酶，山核桃蒲壳 Nutritional conditions for laccase production by Trametes hirsuta D2 in solid-state fermentation using hickory hull YANG Liu* LÜ Dan-Dan DENG Jie-Yong CAO Huan-Ying YE Ming (School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, Anhui 230009, China) Abstract: [Objective] In order to increase the laccase productivity and reduce the production cost, solid-state fermentation (SSF) (using hickory hull) medium for Trametes hirsuta D2 has been studied. [Methods] The effects of supplementation with carbon and nitrogen sources, ratio of carbon and nitrogen source and the content of hickory hull on laccase production were investigated. [Results] The hickory hull was a good support for mycelial growth during laccase fermentation, and the corn flour and rapeseed meal were found efficient and selected as additional nutrition for laccase fermentation. The optimum solid materials in medium