《固相萃取毛细管电泳法检测敌百虫的条件优化》-毕业学术论文（设计）.docVIP

PAGE lw 山东农业大学 毕业论文 题目：固相萃取毛细管电泳法检测敌百虫的条件优化 学 院 食品科学与工程学院 专业班级 食品科学与工程专业4班 届 次 2012届 学生姓名 学 号 指导教师 （副教授） 二〇一二年六月 固相萃取毛细管电泳法检测敌百虫的条件优化 摘要: 固相萃取是一种试样预处理技术，由液固萃取和柱液相色谱技术相结合发展而来。自从出现一次性固相萃取商品柱算起，固相萃取一起现代形式存在已有多年的历史。固相萃取作为制备液体试样优先考虑的方法取代了传统的液液萃取法。毛细管电泳也称为高效毛细管电泳,是近年来发展起来的一类以毛细管为分离通道，以高压直流电场为驱动力的新型液相分离分析方法。是现代分析化学研究的前沿领域之一， 它是利用液体介质中的带电粒子在电场作用下迁移速度不同而进行分离的方法。本文应用固相萃取高效毛细管电泳法对敌百虫的含量进行了测定，研究了缓冲体系、洗脱液最优配比对敌百虫测定的影响。结果表明， 在NaH2PO4 -Na2HPO4缓冲液、甲醇和水配比为80：20的洗脱体系时，敌百虫的测定效果最佳。 关键词：固相萃取；毛细管电泳；敌百虫；条件优化 Solid-phase extraction and Capillary electrophoresis detect of the optimal conditions for trichlorfon. Abstract: Solid-phase extraction is a kind of pretreatment technology, It is developed of liquid liquid-solid extraction and column chromatography technology. Since starting a one-off by solid phase extraction column of goods, solid-phase extraction with modern form has a history of many years. Solid-phase extraction as a priority for liquid sample preparation is instead of a traditional liquid-liquid extraction. Capillary electrophoresis is also known as capillary electrophoresis, is a class developed in recent years as the separation by capillary channel to high electric field-driven phase separation and analysis of new methods of modern analytical chemistry frontier areas. It is the use of liquid media under the electric field of charged particles move at different speeds and separation method. In this paper, Solid-phase extraction and high performance capillary electrophoresis for the content of trichlorfon were determined to study the detection wavelength and the optimal ratio of Washing liquid on the determination of trichlorfon. The results show that 10 mmolL-1NaH2PO4-Na2HPO4 buffer, the proportion of methanol and water is 80:20, the determination has the best effect. Keywords: Solid-phase extraction; Capillary electrophoresis; Trichlorfon; methamidophos optimization. 目录 1 前言…………………