油松花粉离体萌发及萌发特征研究.PDF

中国农学通报 2012,28(28):21-27 Chinese Agricultural Science Bulletin 油松花粉离体萌发及萌发特征研究 田 菊，尚峰男，宋少宇，王 君，康向阳 （北京林业大学林木育种国家工程实验室/林木花卉遗传育种教育部重点实验室，北京100083） 摘 要：为了加强油松有性杂交，为种子园育种研究奠定基础，对油松花粉离体萌发的适宜条件及萌发特 征进行了系统研究。结果表明，油松花粉在无蔗糖培养基中仍具一定萌发能力，蔗糖的添加可显著促进 花粉萌发，但高浓度蔗糖可抑制花粉管的生长；油松花粉管易形成分枝，蔗糖浓度与分枝花粉频率呈正 相关；硼酸的添加在培养初期可有效加速花粉萌发，但过高浓度的硼酸对花粉萌发具抑制作用；Ca2+ 对 花粉萌发的影响较小，不同浓度Ca2+ 处理均可获得较高的萌发率；培养温度对花粉萌发的促进作用明 显，但温度的持续升高将抑制花粉萌发。综合萌发率、花粉管生长及分枝花粉管频率，油松花粉萌发的 最适液体培养基组成为：2.00%蔗糖+0.075%硼酸+0.005% Ca2 + ，26℃下光照培养，72 h 时萌发率可达 (96.10±0.47)% 。 关键词：油松；花粉；离体萌发；花粉管生长 中图分类号：S722.3+4 文献标志码：A 论文编号：2012-2422 InVitroPollenGerminationandCharacteristicsonPollenTubeofPinustabulaeformisCarr. Tian Ju, Shang Fengnan, Song Shaoyu, Wang Jun, Kang Xiangyang (NationalEngineeringLaboratoryforTreeBreeding/KeyLaboratoryofGeneticsandBreedinginForestTreesand OrnamentalPlants,MinistryofEducation,BeijingForestryUniversity, Beijing 100083) Abstract: In order to further research on sexual hybridization and seed orchard breeding in Pinus tabulaeformis Carr., in vitro germination and characteristics of germination on pollen of P. tabulaeformis were studied. The results showed that in medium without sucrose, the pollen still had capability to germinate, although the germination rate was relatively low. The sucrose could promote pollen germination significantly, but high concentration of sucrose inhibited pollen tube growth. Tube branches could be formed during germination, and the frequency of branched pollen grains was positively correlated with sucrose concentration. At early of germination, boron acid was able to accelerate the pollen germination. However, excessively high concentration of the boron acid inhibited germination.