Hp抗菌药耐药性的研究：在我国北京和东南沿海地区资料.ppt

简介 The antimicrobials?resistance?of?Helicobacter pylori?(H.?pylori) was able to sharply decline the eradication rate of H.?pylori?both in adults and?children, but there are limited studies about the?primary?antibiotic resistance?and the related gene mutations, specifically in China. Chinese Center for Disease Control and Prevention studied?antibiotic?resistance?of?H.pylori?isolated?from?Beijing?children and in?the?Southeast?Coastal?Region ?of?China, respectively. BACKGROUND The antimicrobials?resistance?of H.?pylori was able to sharply decline the eradication rate of H.?pylori?both in adults and?children, but there are limited studies about the?primary?antibiotic?resistance?and the related gene mutations, specifically in China. MATERIALS AND METHODS The?primary?resistance?to 9 antibiotics of 73 H.?pylori?strains?isolated?from gastric biopsies of?children?recruited at Beijing?Childrens Hospital was assessed, and the mutations in 23S rRNA gene of 65 macrolide-resistant strains and in gyrA and gyrB of 12 quinolone-resistant strains were investigated. RESEULTS The?resistance?rate to clarithromycin, azithromycin, metronidazole, levofloxacin, moxifloxacin, and rifampicin was 84.9%, 87.7%, 61.6%, 13.7%, 15.1%, and 6.8%, respectively. No?resistance?to amoxicillin, gentamicin, and tetracycline was observed. Dual, triple, and quadruple antibacterial resistant percentage was 46.6% (34/73), 15.1% (11/73), and 2.7% (2/73), respectively. RESULTS The gene mutation rate of A2142C, A2142G, and A2143G in 23S rRNA gene was 1.5% (1/65), 6.2% (4/65), and 84.6% (55/65), respectively. The detection rate of mutations of Asn87, Asp91, and Met191 in GyrA was 41.7% (5/12), 25% (3/12), and 25% (3/12), respectively. CONCLUSIONS The high prevalence of?primary?antibiotic?resistance?was out of expectation in H.?pylori?strains?isolated?from the?children?in?Beijing. Antibiotic?susceptibility should be made clear before the?antibiotic?was used in the anti-H.?pylori?therapy in this populatio