电化学免疫传感器测定牛奶中的青霉素（论文）.pdf

《化学通报》在线预览版 电化学免疫传感器测定牛奶中的青霉素 a a * b 武 海 杨维春 马 洁 a b ( 平顶山学院化学化工学院 平顶山 467002; 首都师范大学化学系 北京 100037) ∗ 摘 要 利用共价键合法, 将新亚甲蓝(NMB)与辣根过氧化酶 (HRP) 标记的青霉素多克隆抗体 (Ab ) 修饰于玻碳电极 表面, 制成电流型免疫传感器；考察了该传感器的电化学行为、对 H2O2 的电化学响应。结果表明，NMB 作为介体能有 效地传递电子, 传感器对 H2O2 具有很好的催化作用。用此传感器对牛奶中的青霉素进行检测, 其线性范围为 0.25～3.00 ng/mL (R ＝0.984), 检出限为 0.298 ng/mL 。 关键词 免疫传感器 新亚甲蓝 共价键合 青霉素 Determination of Penicillin in Milk by the Electrochemical Immunosensor a a ∗ b Wu Hai ,Yang Weichun , Ma Jie ( aCollege of Chemistry and Chemical Engineering, Pingdingshan University, Pingdingshan 467002; bDepartment of Chemistry, Capital Normal University, Beijing 100037) Abstract An amperometric immunosensor was fabricated by co-immobilizing new methylene blue (NMB) and Horseradish Peroxidase (HRP)-labeled penicillin polyclonal antibody (Ab∗) on a glassy carbon electrode through covalent immobilization. The electrochemical behaviors, the response on hydrogen peroxide of the immunosensor had been investigated. The results showed that the NMB enhanced the electron transfer between HRP and electrode and the sensor exhibited excellent catalytic activity to H O . The linear dependence of current on penicillin concentration in milk was in the range of 0.25～3.00 ng/mL with 2 2 the correlation coefficient of 0.984 , the detection limit was 0.298 ng/mL. Keywords Immuno