(沙门氏菌)肉鸽致病菌分离及分子生物学鉴定文献无页码.docVIP

毕业设计论文 肉鸽致病菌的分离与分子生物学鉴定 教学单位: 化学与生物工程学院 专业名称: 生物技术（生物制药） 学 号: 28060101021 学生姓名: 付涛 指导教师: 李雪雁 指导单位: 化学与生物工程学院 完成时间: 2012.4.30 电子科技大学中山学院教务处制发 肉鸽致病菌的分离与分子生物学鉴定 【摘要】 实验通过对患有沙门病的乳鸽病灶样本进行研磨、溶解、稀释、选择培养等操作后，分离出单一的沙门氏菌菌落，对这些菌落进行纯化培养，通过观察菌落形态特征、培养特性结合血清型鉴定结果以及生化实验结果对菌种进行初步判断。通过提取沙门氏菌基因组DNA，利用聚合酶链式反应对病鸽沙门氏菌的16SrDNA片段进行克隆，所得特异性片段以琼脂糖为介质进行电泳并与DNA marker对比确定所克隆DNA片段大小，初步判断是沙门氏菌16SrDNA片段。对片段测序并与GenBank中的序列进行比对，确定获得了鸽子沙门氏菌的分离菌株。 【关键词】 鸽子，沙门氏菌，分离筛选，生化实验，PCR Isolation and Molecular Biology Identification of Pathogenic bacteria from Pigeons 【Abstract】By grinding, dissolution, dilution, selection, cultivation and other operations on pigeon lesion samples with Salmonella disease,the experiment isolates a single colony of Salmonella and these bacteria are purified and cultured. By observing the colony morphology characteristics and cultured characteristics,combined with the result of the theseroty and biochemical test,the preliminary judgment of the strains can be made. By extrating Salmonella genomic DNA and using the polymerase chain reaction on Bingge to clone Salmonella 16SrDNA fragments,with the agarose as a medium,the specific fragment attained from the above is on electrophoresis and the comparation with the DNA Marker,to determine the cloned DNA fragment size,from which the preliminary judgment is the Salmonella 16SrDNA fragment. By sequencing the fragments and alignning them with the sequence in GenBank, pigeon Salmonella 16SrDNA specific sequences are obtained by sequencing of the company. As the result, the separated strains of the Salmonella from pigeon can be carried out through this experimental identification. 【Key Words】Pigeon，Salmonella，Isolation and Screening，Biological and chemical experiments，PCR 目录 TOC \o 1-3 \h \z \u HYPERLINK \l _Toc240537129 第1章前言 PAGEREF _Toc240537129 \h 1 HYPERLINK \l _Toc240537130 1.1中山市肉鸽 HYPERLINK \l _Toc240537131 2 HYPERL