武振龙-乳酸菌与动物肠道健康.pptxVIP

武振龙;;乳酸菌分布与来源;人肠道中的乳酸菌：1%(16S rDNA);仅少数乳酸菌有 益生作用（20%） ;乳酸菌(Lactic acid bacteria, LAB): 肠道中重要益生菌;1950年，FDA首次批准抗生素用作饲料添加剂; 瑞典：1986年首先提出来禁止饲料中添加抗生素; 丹麦：2000年起抗生素在兽医监督下使用 欧盟：2006年全面禁止抗生素作为饲料添加剂 美国：FDA从2014年起用3年时间禁止使用抗生素 中国：2015年底在停用4种喹诺酮类（洛美、培氟、氧氟、诺氟沙星） 2016年11月停止硫酸黏杆菌素的饲料添加剂 2013年我国抗生素使用量为16.2万吨，52%为动物用;Probiotics：lactic acid bacillus Prebiotics Enzymes Natural products Essential oils Immunostimulants Organic acids;J. Agric. Food Chem. 2014, 62, 860?866; ;乳酸菌提高猪的生长性能：断奶仔猪;;（一）提高猪的生长性能：减少腹泻发生;改善蛋品质、降低氨浓度;（二）改善肠屏障功能;（二）改善肠屏障功能：粘蛋白的表达;Anderson et al., 2010;Lactobacillus rhamnosus GG prevents H2O2-induced disruption of tight junction protein in Caco-2 cell monolayers;Yang et al. BMC Microbiology (2015) 15:32 ;（三）维持内环境稳态：抗氧化功能;（三）维持内环境稳态：抗氧化功能;Int J Prev Med 2016;7:102;Lactobacillus fermentum Suo Attenuates HCl/Ethanol Induced Gastric Injury in Mice through Its Antioxidant Effects;（三）内环境稳态：促进细胞增殖;（四）免疫调节作用;Effect of Lactobacillus plantarum B1 on secreted IgA(sIgA) concentration in ileal mucosa;L. plantarum TN8 mitigates the histological damage in distal colon of rats induced with TNBS colitis.;（四）免疫调节作用：细胞因子表达;LGG：positive control ZJ615：Low adhesive strain of Lactobacillus ZJ617：high adhesive strain of Lactobacillus ;Inflammatory cytokines;Figure 3. Effect of various treatments on the gastrointestinal (GI) transit in the mouse model of activated carbon-induced constipation (n=10 per group);Effects of Lactobacillus rhamnosus on chronic-alcohol-induced liver steatosis ;Lactobacillus plantarum FH185 reduced adipocyte size of the epididymal fat pads in mice with diet-induced obesity;Lactobacillus ameliorates fructose-induced hepatic fatty acid accumulation;Effect of Lactobacillus GG on the severity of alcoholic steatohepatitis assessed by the total liver histology, liver necroinflammation scores, liver fat content and liver MPO level;Yvonne Ritze et al., 2014;Mechanisms of action of probiotics in the GI tract;优良乳酸菌株的选育;饲料加工工艺改进：有效活菌数;乳酸菌黏附及作用机理 ;Ackn