切花文心兰花梗芽组培快繁技术研究-广东农业科学.PDFVIP

东农业科学 2015 年第 4 期 23 切花文心兰花梗芽组培快繁技术研究 王安石 韩 松 陈施明 潘英文 林明光 海南出入境检验检疫局热带植物隔离检疫中心袁海南 海口 570311冤 要 以切花文心 南茜 Onc idium Gower Ramsey 为材料袁对以花梗芽为外植体的切花文心 组培快繁 技术进行研究遥 结果表明袁以成熟度 2 外形呈笋状袁花苞和分叉始露出花梗苞片袁高约 70 cm 冤和中部节位的花 梗上的芽为最适宜外植体曰 用 0.1%升汞消毒处理花梗芽的适宜时间为12 min 曰1/2MS +6-BA 2.0 mg/L+T DZ 1.0 mg/L+NAA 0.2 mg/L 为花梗芽最佳初代诱导培养基曰 继代培养以6-BA 3.0 mg/L+NAA 0.3 mg/L 为最佳激素组 合曰以温度 25益和光照强度 2 500 lx 光照时间10~12 h/d 冤的培养条件最为理想遥 关键词 文心 曰 切花曰 花梗芽曰 组织培养曰 快速繁殖 中图分类号 S682.31 文献标识码 A 文章编号 1004-874X 2015 冤04-0023-05 Tissue culture and rapid propagation for Oncidium inflorescence shoots WANG An-shi, HAN Song, CHEN Shi-ming, PAN Ying-wen, LIN Ming-guang (Post-Entry Quarantine Station for Tropical Plant, Hainan Entry-Exit Inspection and Quarantine Bureau, Haikou 570311, China) A bstract: The technology of tissue culture and rapid propagation for Onc idium inflorescence shoots was studied using the cut -flowers of Onc idium Gower Ramsey as test material. The results showed that the shoots on inflorescence at maturity level 2 (bamboo shaped appearance, bud and bifurcation pedicel bracts, about 70 cm) and those in the central section of inflorescence were best suitable as explants. The suitable disinfection time of inflorescence shoots w ith 0.1% mercuric chloride solution was 12 min; 1/2MS +6-BA 2.0 mg/L+TDZ 1.0 mg/L +NAA 0.2 mg/L was the best original induction medium. Successive transfer cultrue took 6-BA 3.0 mg/L+NAA 0.3 mg/L as the best hormone combinations and good ideal culture conditions were at 25益 a