从发酵液中反胶束萃取纯化纳豆激酶影想温度和相体积比.pdf

Bioprocess Biosyst Eng (2006) 28: 267–273 DOI 10.1007/s00449-005-0032-1 ORIGINAL PAPER Jun-Guo Liu Æ Jian-Min Xing Æ Tian-Shi Chang Hui-Zhou Liu Purification of nattokinase by reverse micelles extraction from fermentation broth: effect of temperature and phase volume ratio Received: 26 September 2005 / Accepted: 10 October 2005 / Published online: 8 December 2005 Springer-Verlag 2005 Abstract Nattokinase is a novel fibrinolytic enzyme that However, the presence of nutrients, amino acids, cell is considered to be a promising agent for thrombosis debris and other contaminating proteins makes enzyme therapy. In this study, reverse micelles extraction was recovery from whole fermentation broth a challenge. applied to purify and concentrate nattokinase from The interaction of the surfactant with any broth con- fermentation broth. The effects of temperature and stituents may affect the preferential separation of the phase volume ratio used for the forward and backward desired product. Any complexation of the surfactant extraction on the extraction process were examined. The with cell debris or broth constituents will decrease the optimal temperature for forward and backward extrac- effective surfactant to achieve solubilization and sepa- tion were 25C and 35C respectively. Nattokinase be- ration [2]. By now, only a few proteins, such as alkaline came more thermosensitive during reverse micelles protease [2], a-amylase [3], inulinase [4], xylanase [5], extraction. And it could be enriched in the stripping extracellular acid phosphatse [6], alcohol dehydrogenase phase eight times during backward extraction. It was [7], lysozyme [8, 9] have been reported to be purified found that nattokinase could be purified by AOT r