干扰素治疗对慢性乙肝患者CD4.doc

干扰素治疗对慢性乙肝患者CD4+ CD25+调节性T细胞水平的影 响研究 ［摘要］目的探讨慢性乙型肝炎患者在干扰素抗病毒治疗中 外周血调节性T细胞的变化及临床意义。方法选择2013年1 月?2015年1月在我院行干扰素抗病毒治疗的慢性乙肝患者60 例及同时期体检的健康成人20例，分别于治疗前、治疗后第12、 24、36、48周及停药后24周收集患者的外周血，比较外周血中 CD4+CD25+调节性T细胞(Tregs)血清HBV-DNA载量以及谷氨 酸-丙酮酸转氨酶(ALT)的变化。结果治疗前，完全应答组、 部分应答组以及无应答组外周血CD4+CD254-调节性T细胞比例、 ALT含量以及HBV-DNA载量组间比较差异无统计学意义 (P0. 05),但均显著高于健康对照组，差异有统计学意义 (P0. 05)；治疗后，完全应答组和部分应答组患者各时间段 Tregs比例均较治疗前显著下降(P〈0.05)；在治疗及停药期间, 完全应答组和部分应答组患者的Tregs比例一直在下降;治疗期 间HBV-DNA转阴患者的Tregs比例逐步下降且显著低于未转阴的 患者(P0. 05)；治疗期间血清学转换的患者Tregs比例逐步下 降且显著低于未转换的患者(P0. 05)o结论 慢性乙肝患者在 接受干扰素抗病毒治疗期间及治疗后，其外周血调节性T细胞比 例下降，且越早出现下降的患者其治疗后血清转阴的可能性越 大。［关键词］乙型肝炎；干扰素；调节性T 大。 ［关键词］ 乙型肝炎；干扰素；调节性T细胞; 外周血 ［中图分类号］R512. 62 ［文献标识码］A ［文章编号］ 1673-9701 (2016) 24-0012-04 Effect of CD4+ CD25+ regulatory T cell level of chronic hepatitis B during interferon theraping XUE Haibol MA L订i2 LIN Xianfanl WU Jinmingl 1. The First Department of Gastroenterology, the First Affiliated Hospital of Wenzhou Medical University , Wenzhou 325000, China； 2. Departme nt of Rheuma to logy and Immunology, the First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, China [Abstract] Objective To investigate the changes and clinical significance of peripheral blood regulatory T cells in chronic hepatitis B patients treated with interferon. Methods 60 patients with chronic hepatitis B who were treated with interferon and 20 healthy adults were selected from January 2013 to January 2015 in our hospital. Peripheral blood was collected before treatment and 12th weeks, 24th weeks, 36th weeks, 48th weeks after treatment and 24 weeks after withdrawal, the changes of CD4+CD25+ regulatory T cells (Tregs), serum HBV-DNA and glutamate pyruvate aminotransferase （ALT） in peripheral blood were compared? Results There were no significant differences in the proportion of CD4+CD25+ regulatory T cells, ALT content and HBV-DNA content between complete response group, partial response group and non response group before treatment （P〉0.05）, but they were signif