二氧化碳氧气混合干燥保存周围神经的实验研究外科学手外科专业论文.docxVIP

二氧化碳氧气混合气干燥保存周围神经的实验研究摘 二氧化碳氧气混合气干燥保存周围神经的实验研究 摘 要 目的：通过对大鼠坐骨神经在C02／02中干燥保存的效果和移植后神经再生的情 况的研究，探索C02／02中干燥保存法在周围神经保存中的应用价值。 方法：将F344大鼠坐骨神经15mm分别通过不同方式处理后移植到F344大鼠 坐骨神经12mm缺损处。分组：A：将供体神经不做任何处理，取出后即刻移植； B(干燥保存组)：将供体神经保存在C02／02混合气(P C02=100hPa，P 02=900hPa) 中；C：将供体神经置于UW液中；D(干燥保存组)：将供体神经保存在纯02 (P 02=1000hPa)中，B、C、D组4。C保存3周后移植。移植前，电镜评估移植 段神经纤维横断面结构；移植后12周，通过电生理、肌肉湿重、肌纤维横截面 积、神经免疫荧光染色、神经纤维计数、神经纤维圆当量直径计算、神经横截面 电镜形态学观察、统计学分析等方法评估神经再生情况。 结果：移植前电镜形态学观察移植段神经显示B组Schwann细胞和髓鞘仅轻度 变性，接近于A组正常神经纤维结构，明显优于C、D组。移植术后12周，通 过以上指标评估神经纤维再生效果，B组与A组相近，并显著优于C、D组。 结论：C02／02干燥保存法可成功应用于周围神经组织的保存，C02在保存过程中 发挥了重要作用。 关键词：二氧化碳；同种异体；移植；坐骨神经；保存 二氧化碳氧气混合气干燥保存周围神经的实验研究Abstract 二氧化碳氧气混合气干燥保存周围神经的实验研究 Abstract Background：Carbon dioxide acts as a buffer，thereby decreasing the metabolism of the cells in organs and tissues．This study investigated whether peripheral nerve segments in rats could be preserved with mixed carbon dioxide／oxygen gases in desiccating conditions． Methods：In 48 inbred Fischer(F344)6-week-old male rats，autologous sciatic nerve segments were harvested and preserved in 3 different ways(group B，in mixed carbon dioxide／oxygen gases；group C，in University of Wisconsin solution；and group D，in pure oxygen gas)for 3 weeks at 4。C before being transplanted to bridge sciatic nerve gaps created by the removal of nerve segments in the recipients．Group A nerve segments were transplanted immediately after having been harvested． Results：The viability of the nerve segments WaS assessed with electron microscopy before transplant，and nerve regeneration was evaluated with electrophysiologic， histologic，and morphologic studies 1 2 weeks after transplant．The neural cell viability of the preserved nerve segments was confirmed in group B，and demyelination and the disruption of Schwann cells was revealed in groups C and D． The heterotrophic grafted nerves could be regenerated，and target muscles achieved efiective reinnervation．As a result，the peripheral nerveS were thus integrally preserved in desiccating conditi