响应面法优化吴茱萸五加叶柚皮苷纯化工艺.PDF

·868 · 中草药 Chinese Traditional and Herbal Drugs 第50 卷 第4 期 2019 年2 月 响应面法优化吴茱萸五加叶柚皮苷纯化工艺 肖 珊，罗 姣，张斌贝，高大林，刘向前* 湖南中医药大学药学院，湖南 长沙 410208 摘 要：目的 利用响应面设计法优化聚酰胺树脂纯化吴茱萸五加Acanthopanax evodiaefolius 叶中柚皮苷的工艺。方法 对 上样质量浓度、上柱样用量、洗脱体系、洗脱剂用量、体积流量5 个因素进行单因素考察，并使用响应曲面设计法优化筛选 出聚酰胺树脂纯化吴茱萸五加叶中柚皮苷的最优工艺条件。结果 吴茱萸五加叶中的柚皮苷的最优纯化工艺条件为上柱样质 量浓度为4.0 mg/mL ，上样量体积为3.5 BV，洗脱体系为30% 甲醇，洗脱剂用量为3.0 BV，洗脱体积流量为8.0 BV/h。在该 条件下，柚皮苷质量分数由5.08%提高到56.12%，得率为41.69% 。再经一步重结晶后质量分数达到90% 以上，符合医药原 料要求。结论 聚酰胺树脂对吴茱萸五加叶中柚皮苷的纯化效果好，操作简单，效率高，稳定性好，可用于工业推广。 关键词：响应面法；聚酰胺树脂；吴茱萸五加；柚皮苷；纯化；单因素实验 中图分类号：R284.2 文献标志码：A 文章编号：0253 - 2670(2019)04 - 0868 - 07 DOI: 10.7501/j.issn.0253-2670.2019.04.012 Optimization of purification technology of naringin from leaves of Acanthopanax evodiaefolius by response surface methodology XIAO Shan, LUO Jiao, ZHANG Bin-bei, GAO Da-lin, LIU Xiang-qian School of Pharmacy, Hunan University of Chinese Medicine, Changsha 410208, China Abstract: Objective Response surface methodology was used to optimize the purification process of naringin from Acanthopanax evodiaefolius leaves by polyamide resin. Methods The optimum technological conditions for the purification of naringin in the leaves of Acanthopanax evodiaefolius were screened by single factor investigation and response surface design with five factors, including the concentration of sample, sample loading, the elution system, the amount of eluent, and the flow rate. Results The optimum purification conditions of naringin in the leaves of A. evodiaefolius were as follow: the concentration of the sample was 4.0 mg/mL, the sample volume was 3.5 BV, the elution system was 30% methanol, the eluant volume was 3.0 BV, and the elution flow rate was 8.0 BV/h. Under this condition, the purity of naringin was improved from 5.08% to 56.12%, and the yield was 41.69%. And mass fraction reached more than 90% after recrystallization,