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31 1 () Vol. 31 No. 1
2008 3 JOURNAL OF NANJING NORMAL UNIVERSITY ( N tur l Science Edition) M r, 2008
,
(, 210046)
[ ] (Therm otog a m aritima MS8), ,
. , . TLC
, , . , K,
, T. mari tmi a M S8.
[ ] , , , ,
[ ] Q936 [ ] A [ ] 100 1-46 16( 2008) 01-0088-05
Purification, Chem ical Characterization and SensitiveAnalysis
of the Sheath From ThermotogaMaritimaMS8
Zhou Q ing, Sh oW eil n
( School of L ife Science, N njing Norm lUn iversity, N njing 210046, Ch in )
Abstract:Therm otog a m aritmi a M S8 is strictly n erobic nd extrem ely therm ophilic. The rod-sh ped cells re surroun-
ded w ith she th like structure b lloon ing over the ends of the rods. In contr st to the pp lic tion of centrifug tion lm em-
br ne filter or d i lysis, the m ethod of sucrose isopycnic gr d ient centrifug tion w s m ore su it b le. Through the n lysis
of th in-l yer chrom togr phy, she th w s com posed of proteins, lipids nd s cch ride. It resemb led the ch r cteriz tion
of outerm embr ne. And she th w s sensitive to Protein se K w hile the cell w s still live. Th is findingm y prom ote the
tr nsform tion study ofThermo tog a m ar itmi a.
K ey ords:Thermo tog a m ar itima, extrem ely therm ophile, she th, thin-l yer chrom togr phy, tr nsm ission electon m i-
croscope
(Therm otog a m aritima M S8), 80 ,
90 . , T. m aritmi a M S8
, .
T. maritim a M S8, ,
. 85 , ,
. T. m aritima M S8.
, T. m aritim a M S8, ,
, T. maritmi a M S8.
1
11
CENTREX m icrofilter Sch leicher Schuell; RN se, DN se I, HEPES, S igm ;
: 2007-04-09
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