一氧化氮和过氧化氢诱导水稻细胞过敏反应的协同作用分析-生物通.PDF

中国农业科学 2006,39(1):61-65 Scientia Agricultura Sinica 一氧化氮和过氧化氢诱导水稻细胞过敏反应的协同作用分析 齐放军，高世强，吴茂森，何晨阳 ( 中国农业科学院植物保护研究所／植物病虫害生物学国家重点实验室，北京 100094) 摘要：【目的】本文研究了信号分子一氧化氮(NO)和过氧化氢(HO )对水稻细胞过敏反应(HR)的诱导作用。【方 2 2 法】用溴酚兰染色法定量检测这两种信号分子单独或协同诱导作用下水稻细胞的 HR。【结果】该两种信号分子可 以独立或协同诱导水稻细胞 HR 的产生，HO 对 NO 诱导的 HR 具有调节作用，但 NO 与 H O 浓度平衡与否对 HR 诱导 2 2 2 2 并无影响。【结论】H O 和NO均是诱导水稻细胞HR的信号分子，诱导过程中并不存在 “NO/HO 浓度平衡作用机制”。 2 2 2 2 关键词：NO；H O ；水稻细胞；过敏反应；协同作用 2 2 Analysis of Synergetic Induction of Hypersensitive Response by Nitric Oxide and Hydrogen Peroxide in Rice Suspension Cultured Cells QI Fang-jun, GAO Shi-qiang, WU Mao-sen, HE Chen-yang (State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094) Abstract: 【Objective 】The hypersensitive response (HR) in rice suspension cultured cells induced by exogenous addition of the signal molecules, nitric oxide (NO) and hydrogen peroxide (H O ) was studied. 【Method 】The HR of rice cells induced by NO and 2 2 H O dependently and/or in synergy, was quantitatively determined by the bromphenol blue staining method.【Result 】Results showed 2 2 that HR of rice cells could be induced by NO and H O dependently and/or in synergy and that NO-induced HR could be regulated 2 2 by H O The ba