人参皂苷Rg3对大鼠颈动脉损伤后内膜增生及平滑肌细胞凋亡的影响.PDFVIP

·3082 · 中草药 Chinese Traditional and Herbal Drugs 第49 卷 第13 期 2018 年7 月 人参皂苷Rg 对大鼠颈动脉损伤后内膜增生及平滑肌细胞凋亡的影响 3 陈文明，赵永超，刘围围，龙禹哲，张 赟，石 蓓 遵义医学院附属医院 心血管内科，贵州 遵义 563099 摘 要：目的 研究人参皂苷Rg3 对大鼠颈动脉损伤后新生内膜增生及平滑肌细胞凋亡的影响。方法 40 只SD 大鼠随机分 为假手术组、模型组和人参皂苷Rg3 低、高剂量 （5、10 mg/kg ）组，各10 只，用球囊制备大鼠颈动脉损伤模型，术后次日 ig 给药，假手术组和模型组给予等量的生理盐水；14 d 后取损伤的颈总动脉血管，用苏木精-伊红 （HE ）染色观察新生内膜 组织形态学改变；采用末端脱氧核苷酸转移酶介导的三磷酸脱氧尿嘧啶缺口末端标记 （TUNEL ）法检测平滑肌细胞凋亡； Western blotting 和qRT-PCR 法检测损伤血管凋亡基因Fas 、抗凋亡基因Bcl-2 的表达。结果 与假手术组比较，模型组大鼠 血管新生内膜明显增厚，内膜与中膜面积比明显增加，新生内膜区平滑肌细胞凋亡率显著增加，Fas 、Bcl-2 基因表达明显增 加；与模型组比较，人参皂苷Rg3 低、高剂量组血管内膜增生明显减轻，内膜与中膜面积比明显下降，新生内膜区平滑肌细 胞凋亡率明显增加，损伤血管Fas 基因表达水平明显升高，Bcl-2 基因表达水平明显降低。结论 人参皂苷Rg3 可能通过促 进平滑肌细胞的凋亡，从而减轻球囊损伤后血管内膜的增生。 关键词：人参皂苷Rg ；内膜增生；平滑肌细胞；细胞凋亡；颈动脉损伤 3 中图分类号：R285.5 文献标志码：A 文章编号：0253 - 2670(2018) 13 - 3082 - 05 DOI: 10.7501/j.issn.0253-2670.2018. 13.019 Effect of ginsenoside Rg3 on intimal proliferation and apoptosis of vascular smooth muscle cells after vascular injury in rats CHEN Wen-ming, ZHAO Yong-chao, LIU Wei-wei, LONG Yu-zhe, ZHANG Yun, SHI Bei Deparment of Cardiology, Affiliated Hospital of Zunyi Medical College, Zunyi 563099, China Abstract: Objective To investigate the effects of ginsenoside Rg3 on intimal proliferation and apoptosis of vascular smooth muscle cells after vascular injury in rats. Methods Forty Sprague Dawley rats were divided into four groups randomly including Sham operation group, model group, ginsenoside Rg3 low-dose group (5 mg/kg), and ginsenoside Rg3 high-dose group ( 10 mg/kg). The carotid artery intima injury model was established by inflation balloons. From the next day after modeling, the rats were treated with ginsenoside Rg3 by ig administation in ginsenoside Rg3 groups, and rats in Sham operation group and model group were administered with same amount of normal saline. The injured common carotid arteries were harvested after 14 d a