局部应用辛伐他汀对家兔下颌骨骨折愈合的影响.PDF

局部应用辛伐他汀对家兔下颌骨骨折愈合的影响 刘屹嵩1，2 牛 恒 2 符增涛2 闫征斌1,2 1.大庆油田总医院口腔外科，黑龙江大庆163411;2.佳木斯大学口腔医学院，黑龙江佳木斯154004 [摘要]目的 研究局部应用辛伐他汀对家兔下颌骨骨折愈合的影响，为辛伐他汀在口腔颌面部骨折愈合的临床应 用提供相应的理论依据。方法 选择24只成年雄性新西兰家兔，所有家兔均在左侧下颌骨体处做线性骨折模型 后行钛板钛钉内固定，将实验家兔随机分成实验组和对照组。实验组家兔下颌骨骨折区局部覆盖含有辛伐他汀 的明胶海绵缓释膜，对照组骨折区局部覆盖不含辛伐他汀的明胶海绵缓释膜。分别于术后第2、4、8周处死实验 动物，通过HE染色和免疫组化法分析辛伐他汀对家兔下颌骨骨折愈合的影响。结果 实验组骨痂形成与改建速 率快于对照组；术后2、4周实验组骨形态发生蛋白-2阳性表达率明显高于对照组(P＜0.05)，在第8周时两组差 异无统计学意义(P＞0.05)。结论 局部应用辛伐他汀能促进新骨的形成，加速骨折的愈合。 辛伐他汀;下颌骨骨折；骨折愈合;骨形态蛋白-2 R782 A 1673-7210(2017)01(a)-0024-04 EffectoflocalapplicationofSimvastatinonmandibularfracturehealing inrabbits LIUYisong1,2 NIUHeng2 FUZengtao2 YANZhengbin1,2 1.DepartmentofOralandMaxillofacialSurgery,DaqingOilFieldGeneralHospital,HeilongjiangProvince,Daqing 163411,China;2.SchoolofStomatology,JiamusiUniversity,HeilongjiangProvince,Jiamusi 154004,China [Abstract]ObjectiveToinvestigatetheeffectofSimvastatinonrepairingmandibularfractureinrabbits,inorderto provideatheoreticalbasisfortheclinicalapplicationofSimvastatininthetreatmentoforalandmaxillofacialfracture. Methods24adultmaleNewZealandrabbitsmadeintofracturemodelwithtitaniumplatefixationweredividedinto twogroupsrandomly,includingexperimentalgroupandcontrolgroup.Therabbitsofexperimentalgroupwerecovered bySimvastatininformofslow-releasegelfoamatlocallesionandthoseofcontrolgroupwerecoveredbygelfoam withoutSimvastatinatlocallesion.After2,4,8weeksofsurgery,rabbitswereexecuted.TheeffectsofSimvastatinon bonefracturerepairingwereevaluatedbyHEstainingandimmunohistologicalstaining.ResultsTherateofbonecallus reactionintheexperimentalgroupwasfasterthanthatofcontrolgroup.Theexpressionofbonemorphogeneticprotein 2intheexperimentalgroupwashigherthanthatincontrolgroupat2and4weeksaftersurgery,andtheexpressionof bonemorphogeneticprotein-2intwogr