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李 婷 , 王 铮 , 张海波 , 金莉莉 , 王秋雨 , 马玉珠
(1. , 110036; 2. , 100010)
摘 要: 目的 ()()()
方法 Agilent Zorbax SB-C18 (4.6 mm×250 mm, 5 μm), -(63:37),
280 nm, 1.0 mL/min, 30 ℃, 10 μL结果 0.3∼10 μg/mL ,
, Y=4203.8X -661.95, R=0.9991
3.000.420.54 μg/g结论 ,
,
关键词: ; ; ; ;
Estradiol concentration in Rana oviduct, ovum of Eriocheir sinensis and
ovum of Crucian carp by high pressure liquid chromatography
1 1 1 1 1* 2
LI Ting , WANG Zheng , ZHANG Hai-Bo , JIN Li-Li , WANG Qiu-Yu , MA Yu-Zhu
(1. Life Science , Liaoning University , Shenyang 110036, China; 2. Bei Fang Shan Qi Biological Technology
Co., Ltd. of Liaoning, Shenyang 100010, China)
ABSTRACT: Objective To determine the concentration of estradiol in Rana oviduct, ovum of Eriocheir si-
nensis and ovum of Crucian carp by high pressure liquid chromatography. Methods The chromatographic
column Agilent Zorbax SB-C18 (4.6 mm×250 mm, 5 μm) was used. Chromatographic conditions were as fol-
lows: the mobile phase was acetonitrile-water (63:37), the detection wavelength was 280 nm, the flow rate was
1.0 mL/min, column temperature was 30 ℃ , and sample amount was 10 μL. Results A good linearity were
obtained in the range of estradiol concentration 0.3∼10 μg/mL with the above condition. Its regression equation
was Y=4203.8X -661.95 with the correlation coefficient R=0.9991. The estradiol concentration of Rana oviduct,
ovum
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