高压液相色谱法测定林蛙油_中华绒螯蟹卵和鲫鱼卵中雌二醇的含量.pdfVIP

1 1 1 1 1* 2 李 婷 , 王 铮 , 张海波 , 金莉莉 , 王秋雨 , 马玉珠 (1. , 110036; 2. , 100010) 摘 要: 目的 ()()() 方法 Agilent Zorbax SB-C18 (4.6 mm×250 mm, 5 μm), -(63:37), 280 nm, 1.0 mL/min, 30 ℃, 10 μL结果 0.3∼10 μg/mL , , Y=4203.8X -661.95, R=0.9991 3.000.420.54 μg/g结论 , , 关键词: ; ; ; ; Estradiol concentration in Rana oviduct, ovum of Eriocheir sinensis and ovum of Crucian carp by high pressure liquid chromatography 1 1 1 1 1* 2 LI Ting , WANG Zheng , ZHANG Hai-Bo , JIN Li-Li , WANG Qiu-Yu , MA Yu-Zhu (1. Life Science , Liaoning University , Shenyang 110036, China; 2. Bei Fang Shan Qi Biological Technology Co., Ltd. of Liaoning, Shenyang 100010, China) ABSTRACT: Objective To determine the concentration of estradiol in Rana oviduct, ovum of Eriocheir si- nensis and ovum of Crucian carp by high pressure liquid chromatography. Methods The chromatographic column Agilent Zorbax SB-C18 (4.6 mm×250 mm, 5 μm) was used. Chromatographic conditions were as fol- lows: the mobile phase was acetonitrile-water (63:37), the detection wavelength was 280 nm, the flow rate was 1.0 mL/min, column temperature was 30 ℃ , and sample amount was 10 μL. Results A good linearity were obtained in the range of estradiol concentration 0.3∼10 μg/mL with the above condition. Its regression equation was Y=4203.8X -661.95 with the correlation coefficient R=0.9991. The estradiol concentration of Rana oviduct, ovum