制麦白地霉的筛选及其初步应用-食品与生物技术学报.PDF

第30 卷第2 期 食 品 与生 物技 术学 报 Vol. 30 No.2 2011 年3 月 Journal of Food Science and Biotechnology Mar. 2011 : 1673-1689(2011) 02-0295-07 制麦白地霉的筛选及其初步应用 1, 2 2 2 * 1, 2 乔洪升 , 蔡国林 , 孙军勇 , 陆健 ( 江南大学 工业生物技术教育部重点实验室, 江苏无锡214122; 2. 江南大学生物工程学院, 江苏 无锡214122) : 对40 株白地霉产-葡聚糖酶木聚糖酶 性蛋白酶和纤维素酶的酶活情况进行研究,以 加权评分对这些酶活进行分析评价, 从 选出4 株白地霉菌株(3 号12 号18 号和 39 号) 作为制 麦用微生物通过大麦制麦试验证明:这4 株白地霉均对麦芽的溶解具有促进作用, 其 12 号白 地霉的效果最好与空白对照相比, 制得的白地霉麦芽 , -葡聚糖质量分数降低了17. 8%, 60 in 的滤液体积提高了35.4%,糖化力增加了10.3%, -氨基氮质量分数增加了73%,其他理化指标 也均符合QB1686-2008将12 号白地霉麦芽与对照麦芽分别进行了啤酒酿造实验, 结果表明:白地 霉麦芽的酿造性能优于空白对照麦芽,两种麦芽所酿啤酒的理化指标均符合GB 4927-2008 : 白地霉; 麦; 麦芽; 溶解; 啤酒 :T Q 920. 1 : A Selection of Geotrichum and Its Preliminary Application 1,2 2 2 * 1,2 QIAO Hong-sheng , CAI Guo-lin , SUN Jun-yong , LU Jian ( 1. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China; 2. School of Biotechnology, Jiangnan University, Wuxi 214122, China) Abstract: The activities of -glucanase, xylanase, protease and cellulase produced by 40 strains of Geotrichum cand idum were studied in this anuscript. The weighted ark ethod was used to analyze activities of the above enzy es, and four Gcandid um strains including No. 3, No. 12, No. 18 and No.39 were selected as the alting icrobial strains. The alting trials indicated that four Gcandidum strains could i prove the odification of alt and No12 was the best one. Co pared with that of the control, -glucan content in final alt produced by Gcandidum No12 was decreased by 178%, an