全基因组分析玉米MuDR转座因子插入突变体库-作物学报.PDF

作物学报 ACTA AGRONOMICA SINICA 2011, 37(5): 772−777 /zwxb/ ISSN 0496-3490; CODEN TSHPA9 E-mail: xbzw@ DOI: 10.3724/SP.J.1006.2011.00772 Genome-wide Analysis of MuDR-related Transposable Elements Insertion Population in Maize 1 1 2 2 1 1,* FENG Jing , FU Xue-Qian , WANG Ting-Ting , TAO Yong-Sheng , GAO You-Jun , and ZHENG Yong-Lian 1 National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China; 2 Hebei Research Station of National Key Laboratory of Crop Genetic Improvement, Agricultural University of Hebei, Baoding 071000, China Abstract: Insertional mutagenesis has now been widely used to knockout genes for functional genomics. The maize Mutator transposons hold an advantage of high activity to construct large mutant libraries. In this study, a MuDR line was used to cross with an elite Chinese maize inbred line Z31. A total of 1 000 M individuals were planted and self-pollinated to generate their M fami- 1 2 lies. Experiments were conducted to investigate the insertion specificity of MuDR related transposable elements. Six hundred and ninety-five MuDR inserted flanking sequences were isolated with a modified MuTAIL-PCR method and analyzed with bioinfor- matics. Three hundred and seventy-four non-redundant insertion sites were identified and 298 of them were mapped to a single locus on the integrated maize map. The results revealed some prominent features of the MuDR-related insertions of maize: random dis- tribution across the 10 chromosomes, preferential insertion i