英文文献报告精选课件.ppt

南京林业大学 * 配色方案修改： 配色方案在【格式】--【幻灯片设计】--【配色方案】--【编辑配色方案】下调整。 LOGO的添加： Logo添加修改在【视图】--【母版】--【幻灯片母版】下调整。直接选择logo图片删除或修改。 字体格式的设置： 括标题和文本格式的设置在【视图】--【母版】--【幻灯片母版】下调整。 * rcbL：核酮糖-1,5-二磷酸羧化酶/加氧酶 大亚基 利用葡萄糖可以大量提高混合培养时小球藻中脂含量。仅仅增加葡萄糖5g/l就可以提高脂含量103mg/g（葡萄糖）。比另两种微藻混合培养时提高比例要高一个数量级。 在脂含量最高时对应的葡萄糖含量10g/l，增加的脂含量也达到80mg/g（葡萄糖）。 Reading Report The effect of mixotrophy on microalgal growth, lipid content, and expression levels of the pathway genes in Chlorella sorokiniana 坚持 IF=3.425 坚持 主要内容 本文围绕生物能源微藻的培养进行研究。对目的微藻进行培养优化，对微藻的光合自养、异养和混合培养对其生长、脂质影响进行比较和分析，认为混合培养即可以大量促进微藻的生物量的累积并提高微藻的脂含量，而且代价较异养要低，可以作为微藻大规模培养的可选择手段。且C. sorokiniana CCTCC M209220要优于另外两种微藻。 本文通过实时PCR，对（accD、acc1、rcbL）基因在C. sorokiniana CCTCC M209220中的表达量进行测试，并将表达差异和脂质含量进行比较，选出了和脂质含量密切相关的基因作为将来基因改造的候选基因。认为在非异养条件下，accD基因的表达水平可作为评估脂质含量的检验手段之一。 坚持 Contents Introduction 1. Results 2. Discussion 3. Benefits 4. 坚持 Introduction The search of an improved raw material is one of the major objectives in biodiesel research. Microalgae have many advantages for the production of biodiesel compared to other oil crops. 坚持 Introduction The cultivation of microlagae does not require large areas of arable. Advantages Microalgae can grow rapidly, and many microalgae species are rich in oils. 坚持 Introduction Heterotrophy Mixotrophy Photoautotrophy Without organic carbon sources. Slow growth Lowest cost Relay on organic carbon sources Fast growth Highest cost complement photoautotrophy with organic material Fast growth Relatively cheap 坚持 Mixotrophic Mixotrophic cultivation can complement photoautotrophy with organic material, thus shortening the growth cycle, increasing biomass yield, and decreasing the cost of heterotrophic microalgae cultivation. Introduction 坚持 Acetyl-CoA carboxylase (ACCase) In the branch point, acetyl-CoA carboxylase (ACCase) can catalyze acetyl-CoA to be converted to malonyl-CoA and to enter the pathway of lipid biosynthesis. This reaction is a critical step in fatty acid biosynthesis, so ACCase is consid