基于碳基纳米材料-鲁米诺的电致化学发光生物酶传感新方法分析-分析化学专业论文.docxVIP

Ab Abstract PAGE PAGE IV ABSTRACT In this thesis, the basic principles, condition, main reaction types of electrogenerated chemiluminescence (ECL) were reviewed, moreover, the application was reported about the ECL emitter connected to nanomaterial for biosensor analysis. Graphene quantum dots (GQDs), the recently emerging carbon-based materials, have been conducted as cathodic ECL reagent for biosensing. Electrochemiluminescence resonance energy transfer (ECL-RET) has been used for DNA detection, cytosensing, and immunoassay. In this work, this thesis mainly focuses on constructing novel electrochemiluminescence biosensors for the DNA adenine methylation methyltransferase assay and protein kinase activity detection and the related inhibitors research by utilizing nanomaterials-based signal enhancement. The main works was composed of the following two aspects： Herein, we firstly synthetized GO/AgNPs/luminol composites as ECL reagent and developed a novel ECL biosensor for sensitive DNA adenine methylation (Dam) methyltransferase (MTase) assay. The alkynyl functionalized GO/AgNPs/luminol composites were assembled on the azide-terminated dsDNA modified electrode surface via click chemistry for ECL signal production. Once the Dam and Dpn I (a site-specific endonuclease cleaving the duplex symmetrical sequence of 5′-GAmTC-3′) were added to the above electrode, GO/AgNPs/luminol composites released from the electrode surface to the solution, leading to significant decrease of the ECL signal. Therefore, we can utilize this strategy for detecting with Dam activity in the range of 0.1 to 20 U/mL, and the detection limit was calculated to be 0.03 U/mL. This method can be successfully used in the assay of Dam MTase inhibitors and the inhibition efficiency of 5-fluorouracil was the most serious one, moreover, the IC50 of 5-fluorouracil was calculated to be 18.2 μM. Based on these, this method can be used for the screening of antimicrobial drugs and has a great potential to b