基于脱细胞猪角膜基质的组织工程全层人角膜的体外重建、鉴定及其在动物移植中的作用研究-细胞生物学专业论文.docxVIP

基于脱细胞猪角膜基质的组织工程全层人角膜的体外重建、鉴定及其在动物移植中的作用研究 基于脱细胞猪角膜基质的组织工程全层人角膜的体外重建、鉴定及其在动物移植中的作用研究 中国海洋大学博士研究生学位论文 中国海洋大学博士研究生学位论文 PAGE VII PAGE VII 万方数据 VI万方数据 VI 万方数据 In vitro Reconstruction and Characterization of Tissue-Engineered Human Complete Cornea Using Acellular Porcine Corneal Stroma and Its Function in Animal Transplantation Abstract Cornea is a kind of the transparent connective tissue located in the front of eye, with barrier and refractive function. For various diseases caused by the bodys internal and external factors such as poor development of congenital cornea, corneal scarring, infection or severe corneal perforation, corneal thermal burns and corneal opacity affect the eye sight restoring, penetrating keratoplasty is the only therapeutic approach. However, the number of donor cornea is currently a serious shortage of the necessary, can not meet the clinical needs. With the development of corneal tissue engineering, tissue-engineered human cornea (TE-HC) is expected to become a donor cornea equivalent alternatives to solve the problem of insufficient donor for corneal blind patients to bring sight hope. The core elements of reconstruction of TE-HC in vitro include two aspects. On the one hand, we are capable to get a huge amount of seed cells with great proliferative capacity and security, on the other hand, we can get ideal scaffold with good biocompatibility. Currently seed cells for TE-HC are mainly embryonic stem cells (ESCs), adult stem cells (ASCs) and the transfected cell line cells. Biological scaffold materials include natural biomaterials and synthetic polymer biomaterials. The applications of ESCs lead to ethical controversy, the differentiation rates of ASCs are very low and the transfected cell line cells exist tumorigenic risk, these factors limit the use of stem cells and cell lines transfected cells in the cornea tissue engineering. Application of collagen as natural biomaterial scaffold with insufficient in terms of mechanical properties, as