基于微藻培养技术的废水资源化利用研究-微生物学专业论文.docxVIP

Abstract Microalgae are auxoautotrophs which are high efficiency in photosynthesis, widely distributed, rapidly multiply and highly valueable. Because of unique advantages, microalgae have been regarded as third-generation feedstock for biodiesel production which could replace fossil fuel in the further. However, the culture cost and sustainable raw material supply are still the greatest bottlenecks that restrict large-scale biodiesel production from microalgae. Therefore, except for the screening and cultivation of microalgae for biodiesel and large-scale culture techniques, wastewater recycling and nutrients reusing become the key approaches to reduce cost. In this paper, water samples came from secondary effluent in Baotou northern suburban water purification plant, and microalgae were separated, screened and identified. Wastewater was reused to culture lipid-rich microalgae and vegetables. The feasibility was analyzed, characteristics of growth and lipid accumulation of XJ002 in municipal wastewater were investigated to provide basis for utilization of wastewater resource. The main contents and results of this study are as follows: Four algae strains were obtained from water samples by the plate dilute methods. With morphology and 18S rDNA molecular identification, No.XJ001, XJ002, XJ006, XJ008 strains were identified belonging to Nitzschia, Scenedesmus, Nannochloropsis, Chlorococcum respectively. XJ002 was cultured with municipal wastewater, the effect of sterilization of wastewater, content and composition of nutrients supplementation on the growth and lipid accumulation of microalgae were investigated. Results indicated that XJ002 could grow in the crude municipal wastewater, and biomass yield of microalgae were increased under the conditions of supplementing 50% and 75% BG-11 nutrients. Except for Ca2+ and trace mental solution, Fe3+, NaNO3, K2HPO4·3H2O, MgSO4·7H2O and Na2CO3 could significantly influence (P ＜ 0.05) the growth and lipid accumulation of mi