光合细菌利用乙酸、丙酸、丁酸产氢的研究-应用化学专业论文.docxVIP

IV IV of inoculation was 10% with the hydrogen yield of 13.4% from butyric acid. The optimum sodium glutamate concentration was 8-9mmol/L with the hydrogen yield of 27.3 % when the acetic acid concentration was 40mmol/L . As the butyric concentration was 45mmol/L, the optimum sodium glutamate concentration was 10mmol/L with the hydrogen yield of 11.06% . The suitable pH value was 6.80 in the acetic and butyric acid culture medium, while it was 7.6-7.7 in propionic acid. The hydrogen yield at 34 °C was higher that that at 30 °C for acetic acid, while it was in reverse order for butyric acid. The hydrogen production by Rhodopseudomonas capsulata from propionic acid was remarkably affected by the concentration of metal ions. The suitable level of MgSO4 was 0.1g/L, beyond which the hydrogen production ability from propionic acid would decline dramatically. With MnSO4 of 2.77mg/L, the hydrogen quantity increased 1.02 times and the hydrogen yield reached 19.59%. With ZnCl2 of 0.2mg/L, the hydrogen yield reached 24.73% and the hydrogen quantity increased 2.5 times compared to the non-ZnCl2 formula. At the suitable concentration of CuCl2 of 12.7μg/L, the cumulative hydrogen production and hydrogen yield reached 1395ml/L and 19.08% respectively, with the hydrogen quantity increase of 1.62 times compared to the non-CuCl2 formula. Ni2+ oppress the hydrogen production by Rhodopseudomonas capsulata. The suitable level CoCl2·6H2O is 0.1mg/L, Fe3+ can increase the hydrogen quantity remarkably, With FeCl3·6H2O of 5.4mg/L, the hydrogen quantity increased 6.4 times compared to the non-FeCl3 formula. The investigation of hydrogen production by Rhodopseudomonas capsulata volatile fatty acids showed that the hydrogen production rate had the order of acetic acid propionic acid butyric acid. The duration of hydrogen evolution had the order of propionic acid butyric acid acetic acid. Specifically. It was found that ammonia inhibition disappeared and Rhodopseudomonas capsu