关节内注射臭氧对兔骨关节炎软骨作用的实验研究-骨外科学专业论文.docxVIP

广州医学院硕士学位论文关节内注射臭氧对兔骨关节炎软骨作用的实验研究 广州医学院硕士学位论文 关节内注射臭氧对兔骨关节炎软骨作用的实验研究 PAGE PAGE 5 histological study of cartilage in HE staining,getting the Mank’s score in toluid ine staining and the expression of Matrix Metalloproteinases-1(MMP-1) with immunohistochemistry . Result: RBC :The number of RBC had no significant difference among the four groups（p＞0.05）； ESR: The differences of ESR among the four groups were not statistically significant（p＞0.05）. SOD : The differences of the content of SOD in the blood of group N, A, L and H were not statistically significant（p＞0.05）. The content of SOD in the washing liquid in the joint of group A,L and H were significantly higher than the N group（P0.05）; The content of SOD of groups increased by degree from group N, A, L to H，the differences among the groups were statistically significant（p＜0.05）. visual inspection of articular cartilage : Great degeneration of cartilage of medial tibial plateau can be seen in group A, L and H. Group L, H were more serious than group A，and group H was the most serious. nkin’s score : The Mankin’s score of group A, L and H were significantly higher than that of group N; The scores of groups increased by degree from group A，L to H，the differences among the three groups were statistically significant（p＜0.05）. (6)：MMP-1 expression: The expression of MMP-1 in group A, L and H were higher than group N（P0.05）. The expression of MMP-1 increased by degree from group A, L to H, the differences among the three groups were statistically significant （p＜0.05）. Conclusion: Repeated injection of Medical ozone to joint cavity: (1) have little effect on the number of RBC, the ESR and the content of SOD of the blood（; 2）can increase the content of SOD in the joint washing liquid and the increase level of the joint washing liquid appeared to be ozone dosage dependent;（3）may have an adverse deleterious effect on OA-like articular cartilage; （4） increase the e