海南红树林内生拟盘多毛孢多样性及分子系统学研究-植物病理学专业论文.docxVIP

摘要 物种多样性指数与该植物的内生拟盘多毛孢的均匀度指数无关，而与丰富度指数 成正相关。 3. 内生拟盘多毛孢形态学指标－中间色胞颜色在分类中作用的重新评价 本文对分生孢子的中间色胞的颜色进行了系统的研究，通过观察中间色胞 颜色在继代培养的变化，发现分生孢子中间色胞颜色类型应该包含两种类型： “浅褐色-橄榄色”和“褐色-煤烟色”，而非“同色”和“异色”，中间色胞的 颜色在继代培养中是稳定的。同时，用 26 个拟盘多毛孢种的 45 个菌株的 ITS 序 列和 beta-tubulin 基因序列建立分子系统发育树。在系统发育树上，拟盘多毛孢 的 45 个菌株分成 2 大类群(X 和 Y)，和分生孢子有色胞的颜色类型“浅褐色-橄 榄色”和“褐色-煤烟色”相对应。形态学和分子系统学数据都说明中间色胞的 颜色类型在该属的分类中具有非常重要的地位。 关 键 词：红树林，内生拟盘多毛孢，多样性，分子系统学 论文类型：基础研究 II 摘要 Subject: Diversity of Mangrove Endophytic Pestalotiopsis in Hainan and Molecular Phylogenetics of pestalotiopsis Specialty: Plant Pathology Name: Yang Zhang Supervisor: Xiaomin Lin Airong Liu ABSTRACT Pestalotiopsis were a major group of endophytic fungi. There were 235 Pestalotiopsis species were recorded of which were plant pathogens mostly. There have fourteen seven species of Pestalotiopsis been recorded as endophyty since Espinosa-Garcia et al. firstly reported Pestalotiopsis funerea as an endophytic fungus in Sequoia sempervirens in 1990. As a part work of the National Natural Science Foundation of China Grants item “Resources and diversity of endophytic Pestalotiopsis in Mangrove and taxonomic study for Pestalotiopsis” , the investigation of endophytic Pestalotiopsis diversity for Mangrove plant in Dongzhai gang Mangrove Nature Reserve in Hainan was carried out, ITS and beta-tubulin gene sequences were amplified for selected representative strains and Appraisement of several morphologic target for their phylogenetic significances in the taxonomy of genus Pestalotiopsis were explored. Main research contents were showed in the following: Species of mangrove plant endophytic Pestalotiopsis in Hainan In a survey of endophytic Pestalotiopsis species in mangrove plants in Dongzhai gang Mangrove Nature Reserve of Hainan province from 2007 to 2008, a total of 77 Pestalotiopsis species belonging to 28 families were isolated from 11 mangrove plants. Diversity of mangrove plant endophytic Pestalotiopsis in Hainan Colonizatio