哈萨克羊和中国美利奴羊DRB1基因SSCP多态性与细粒棘球蚴病遗传抗性的关联分析-动物遗传育种与繁殖专业论文.docxVIP

哈萨克羊和中国美利奴羊 哈萨克羊和中国美利奴羊 DRB1 基因 SSCP 多态性与细粒棘球蚴病遗传抗性的关联分析 II II Abstract Echinococcus granulosus disease (Cystic Echinococcosis, CE), also known as hydatid disease, is Echinococcus (Echinococcus granulosus, Eg) of the larvae (continued sash phase) parasites in the liver, lungs and other organs of the intermediate host (human and herbivores such as cattle, sheep)，and develops cysts as a zoonotic disease.Xinjiang is Chinas important production base of livestock, echinococcosis in Xinjiang has become the most severe local epidemics to human health, which has caused great losses in animal husbandry. In recent years, the world in studying hydatid disease has been developed to the level of cell and molecular biology,and made a great achievements, the incidence rate also has decreased. But fundamentally control and eradication of the disease is very difficult. Major histocompatibility complex (major histocompatibility complex, MHC) is tightly linked, highly polymorphic loci consisting of complex, widely distributed in vertebrates. MHC molecule mainly combined with the foreign antigen and antigen presented to these T lymphocytes, ultimately result in the immune response. Therefore, many diseases, susceptibility, immune response and production performance are closely related to MHC. As Candidate gene of disease resistance and susceptibility, MHC has become the focus of molecular studies of genetic immunization. MHC-DRB1 gene polymorphism of hazakh Chinese Merino sheep (Merino type) has been studied in this study . and also explore the relevance between MHC-DRB1 gene and hydatid disease, lay the foundation for early in the use of marker-assisted selection of new varieties breeding and further study the resistance mechanism of MHC. 1. In this study, nested PCR-SSCP method was used to study the polymorphism of MHC-DRB1 exon 2 in 200 Kazakh sheep and 205 Chinese Merino sheep (which have 100 and 105 sheep infected with hydatid disease,respectively). Results showed that MHC-DRB1 e