广藿香油治疗阴道炎的实验研究-方剂学专业论文.docxVIP

成都中医药大学硕士学位论文 成都中医药大学硕士学位论文 万方数据 万方数据 中文摘要 目的：探讨广藿香油治疗阴道炎的作用，并进一步探讨其可能的机理。 方法：采用倍比稀释法测定广藿香油对白色念珠菌的体外抑菌活性；构建 小鼠白色念珠菌阴道炎模型，给予广藿香油治疗后，观察模型小鼠阴道分泌物 菌落数和病程的变化；构建家兔白色念珠菌阴道炎模型，给予广藿香油治疗后， 观察家兔阴道分泌物菌落数、阴道念珠菌转阴率和阴道粘膜组织的病理变化。 采用 96-孔板微量稀释法测定广藿香对阴道毛滴虫的活性，并采用透射电镜分别 观察和分析虫株在 1/2 MLC 药物作用 1h、3h 和 5h 后虫株超微结构的变化。 结果：广藿香油对白色念珠菌有良好的体外抑菌活性， MIC 为 0.07～ 0.27mg/ml，且对阳性对照药克霉唑无效的 3 株病原菌也有良好的抑菌活性；广 藿香油能明显减少模型小鼠的阴道菌落数，明显缩短病程；广藿香油能减少模 型家兔的阴道菌落数，提高阴道念珠菌转阴率，减轻家兔阴道粘膜组织损伤。 广藿香油对阴道毛滴虫具有明显的杀虫活性，对来自于临床阴道炎患者分泌物 中的 2 株阴道毛滴虫的最低杀虫浓度（MLC）为 0.41 mg/ml；广藿香油作用后阴 道毛滴虫超微结构较正常对照出现明显变化，并能通过对细胞核和细胞器结构 的破坏，最终杀灭虫体。 结论：广藿香油在体外对白色念珠菌有很好的抑菌活性，在体内广藿香油 能有效抑菌杀菌，并有较好的抗炎消肿作用，对白色念珠菌阴道炎模型小鼠和 家兔有很好的治疗作用，有成为治疗念珠菌性阴道炎新型药物的潜力。广藿香 油具有杀灭阴道毛滴虫的作用，可用于治疗滴虫性阴道炎。 关键词：广藿香油；阴道炎；白色念珠菌；阴道毛滴虫；超微结构变化 iii ABSTRACT Objective: To observe the effect of Patchouli oil in treating vaginitis, and to investigate its possible mechanisms. Methods: Minimum inhibitory concentrations (MICs) of Patchouli oil against Candida albicans were determined by agar dilution method;To construct the mice model of candidal vaginitis, and observing the Patchouli oil on mice model of candidal vaginitis colony number and durttion of effect；Establish the rabbits model of candidal vaginitis, and observeing the Patchouli oil on the number of colonies of Candida albicans vaginitis in rabbits vaginal and the overcast rate of the rabbits model of candida vaginitis and the symptoms of rabbit vaginal mucosa.96-well microdilution method was used to determine the activity on Patchouli oil against Trichomonas vaginalis, and TEM was used to research the ultrastructure changes of Tv2 affected by 1/2MLC Patchouli oil for 1h, 3h, 5h. Results：Patchouli oil had a good antibacterial activity against Candida albicans, MICs were 0.07 ~ 0.27mg/ml, Patchouli oil on the three pathogens have good antibacterial activity which clotrimazole did not have antibacterial activity；Compared with the model group, Patchouli oil could significantly reduce the number of colonies of C