瓜蒌籽油提取及多不饱和脂肪酸的富集与微胶囊制备-食品科学专业论文.docxVIP

PAGE PAGE IV microcapsules of PUFA were made via colloid mill encapsulation process and spray drying method. The yield of microcapsules as indicator and the optimization of encapsulation by orthogonal experiments were made. The results showed that, the highest yield was 84.6% when β-CD: gelatin 7:1, core-wall ratio 1:4, the total solid consistency 25% and grind 6 times. The microcapsules we got were oyster white, exquisite, symmetrical, dry well-distributed and without peculiar smell. The oil content was 15.4% outside of microcapsules was 15.4% and 84.6% inside. The ratio of water content was 3.80%.We found that the microcapsules we got was smooth and without rips ,and the diameter was averaged out at 5～10μm observed by SEM. The photos made by DSC and FT-IR showed that the microcapsules we got were kinds of inclusion complex with PUFA by β-CD and gelatin. The release rate of PUFA from microcapsules in vitro was over 60% in 4 hours. Key words: Trichosanthes kirilowii Maxim. seed; polyunsaturated fatty acid; enrichment; encapsulation 目录 摘要 I AbstractII 1 文献综述 1 1.1 栝楼研究进展 1 1.1.1 栝楼概述 1 1.1.2 国内外研究概述 1 1.1.3 瓜蒌籽的化学成分 2 1.1.4 瓜蒌籽的药理作用 3 1.2 多不饱和脂肪酸研究进展 4 1.2.1 多不饱和脂肪酸概述 4 1.2.2 多不饱和脂肪酸生理功能 4 1.3 多不饱和脂肪酸富集方法研究进展 5 1.3.1 超临界 CO2 流体萃取法 5 1.3.2 尿素包合法 5 1.3.3 分子蒸馏法 5 1.3.4 银离子络合法 6 1.4 微胶囊研究进展 6 1.4.1 微胶囊概述 6 1.4.2 微胶囊的制备方法 6 1.4.3 微胶囊在食品中的应用 8 2 引言 10 3 材料与方法 11 3.1 原料及预处理 11 3.1.1 原料 11 3.1.2 原料预处理 11 3.2 仪器与药品试剂 11 3.2 1 实验仪器 11 3.2.2 实验药品试剂 11 3.3 瓜蒌籽基本成分测定和瓜蒌籽油品质分析 11 3.3.1 瓜蒌籽基本成分测定 11 3.3.2 瓜蒌籽油脂肪酸组成成分分析 12 3.3.3 瓜蒌籽油部分化学特性测定 12 3.4 瓜蒌籽油提取工艺优化 12 3.4.1 单因素试验 12 3.4.2 二次正交旋转试验 13 3.5 尿素包合法纯化瓜蒌籽油 PUFA 的研究 13 3.5.1 混合脂肪酸的制备 13 3.5.2 PUFA 的尿素包合 14 3.6 微胶囊制备工艺研究 15 3.6.1 微胶囊的制备 15 3.6.2 微胶囊产品质量评价及表征 16 4 结果与分析 19 4.1 瓜蒌籽基本营养成分测定和瓜蒌籽油基本品质分析 19 4.1.1 瓜蒌籽基本营养成分测定 19 4.1.2 瓜蒌籽油脂肪酸组成成分 19 4.2 瓜蒌籽油提取工艺优化 23 4.2.1 单因素试验 23 4.2.2 二次旋转正交试验 26 HYPERLINK \l _TOC_250001 4.3 尿素包合法纯化瓜蒌籽油 PUFA 的研究 29 4.3.1 单因素试验 29 4.3.2 尿素包合工艺