海藻酸钙微胶珠培养神经干细胞-化学工程专业论文.docxVIP

海藻酸钙微胶珠培养神经干细胞Culture 海藻酸钙微胶珠培养神经干细胞 Culture of Neural Stem Cells in Calcium Alginate Microbeads Abstract Neural stem cells mSCs)with the capacity of self_renewal and differentiation into neurons and glial cells,have gotten more and more worldwide attention，The finding． research and application ofNSCs will play an important role in the nerve disease treatment and nerve injury reparation．The shortage ofthe source and the number ofNsCs．however,is the main chajlenge for its clinic application． Large—scale three-dimensional culture of NSCs in vitro provides a feasible answer for this problem．But the shear stress in bioreactors carl cause serious cell damage especially for 也e shear sensitive cells like NSCs．To avoid the shear stress．encapsulation of NSCs and then cultivation in bioreactors is thus worth investigating．Therefore，we explored the method ofculturing NSCs in calcium alginate microbeads(Ca-Alg Mgs)． To provide the optimum growth conditiom for NSCs in Ca-Alg MBs．ne gelation parameters,such aS the diameter，reactant concentration，and gelation time should be determined firstly to form the proper beads structure for cell growth．In the experiment different Ca-Alg MBs were formed in different gelafion conditions．Then,the diffusion experiment Was performed tO get the diffusion data for different Ca-Alg 1VIBS．After that，a diffusive mathematical model was set up to find the diffusion coefficient(DAB)ofglucose in Ca-Alg MBs by fitting the experimental data．Base Oil the orthogonN test．the optimum gelation conditions for preparing microbeads with a proper diffusivity were determined．And the gelation parameters are 2 rrlm in beads diameter,1．5％(㈣sodium alginate，35％ (wtO／‘)CaCl2，and the gelation time of 1 0 min． The biocompatibility of Ca-Alg MBs for NSCs WaS tested by coculmring NSCs with Ca-Alg MBs up to 21 days．1he ceils were collected and assayed with the immunocytochemical methods．The results indicate that Ca-Alg MBs have a good biocompatibility for NSCs．After that the c