海南黄灯笼辣椒遗传转化体系建立及cmvcp基因的转化-生物化学与分子生物学专业论文.docxVIP

AbstractPepper((：缸矽5fc“胗l Abstract Pepper((：缸矽5fc“胗l a胛门““，”L)is one of the most important Vegetables fbr its rich nutrient with f}dit and economic value．SeVerely attacked by many diseases， it has being studied in order to improVe its resistance．Biotechn0109y brings us the new way to get to I the target， for example， by transfeHing the disease-resistant 99ne into pepper．In recent years， many researchers haVe done pepper genetic n’ansf．onnation， but little success． The biggest problem in pepper transfbnnation is the di佑culty on the regeneration of the transgenic plants．We made a systematic study on the e日、ect of medium components and physi0109y condition of explants on plant regeneration， and established the system of pepper((：矗psfc“，押c向f聆e甩s8 Jacq．)plant regeneration．Following these w9rk， we studied some conditiOns and methods of pepper transf．ormation， and established the system of pepper genetic transfbrmation The regeneration capabilities of cotyledon with di腩rent age are di圩erent and the cotyledon with 1 2-1 6 days 01d giVes the hi曲est rate of di疵rentiation．1 2-1 6 days 01d is reconlIllended to get explants．Hormone plays an important role in pepper regeneration． 6．BA is better than zT for difrerentiation，and 5mg／L 6-BA+1 mg／L IAA giVes me h培hest rate of difI’erentiation．AgN03 plays an imponant role in controlling the browning of cotyledon explants，and 6mg／L AgN03 is recommended．5m∥L 6-BA can’t result in the elongation of shoots， and 3 mg／L6-BA is recommended． GA3 is necessary for the elongation of shoots and 4mg／L GA3 is recommended．By systematic studies，we found the 芒 high emcient mediums f-6r pepper(C9psfc“2 c向f行P，?sP Jacq．)regeneration：difrerentiation medium(MS+5mg／L 6BA+1 mg／L IAA+6mg／L AgN03)；elongation medium(MS+3 m∥L 6BA+1 m∥L IAA+6mg／L AgN03+4mg／L GA3)；rooting medium(MS+IAA 0．5 m∥L) pBI 1 2 1／CMVcp was constructed and then transf．ormed to彳罟_，口6日c，Prf甜2，“，打q／白cfP玎s EHA1 05．cotyledon was nrst pre—culIured on shoot regeneration medium for 2 days，then c