液质联用法分析雾灵乌头不同部位生物碱成分研究-国际中医中药杂志.PDF

国际中医中药杂志2018年7月第40卷第7期 Int J Trad Chin Med, July 2018, Vol. 40, No.7 ·633 · ·实验与方药 · 液质联用法分析雾灵乌头不同部位 生物碱成分研究 郭晓宇 张伽妹 全庆华 姬瑞芳 谭鹏 刘永刚 【摘要】 目的 应用高效液相色谱-电喷雾-离子阱质谱法分析雾灵乌头不同部位提取物的生物碱。 方法 采用 Agilent XDB-C18 色谱柱(250 mm ×4.6 mm ，5 μm)，以 0.1%氨水-乙腈溶液为流动相，梯度洗 脱，流速为 1 ml/min，柱温为 30 ℃；正离子模式采集。结果 雾灵乌头根部发现 61 种生物碱，鉴别出 46 种。在茎中发现 38 种生物碱，鉴定出 33 种，有 27 种与根部相同。在叶中检测出 18 种生物碱，其中 8 种与根部生物碱相同。结论 该方法精确、可靠、高效，适用于雾灵乌头成分的快速鉴定，为雾灵乌头 的开发利用及阐明其药效物质基础提供参考。 【关键词】 乌头属；生物碱类；质谱分析 Study on the alkaloids in the different parts of Aconitum paniculigerum Nakai var.wulingense (Nakai) by HPLC-MS * Guo Xiaoyu , Zhang Jiamei, Quan Qinghua, Ji Ruifang, Tan Peng, * Liu Yonggang. Master of Grade 2017, School of Chinese Materia Medical of Beijing University of Chinese Medicine, Beijing 102488, China Corresponding author: Liu Yonggang, School of Chinese Materia Medical of Beijing University of Chinese Medicine, Beijing 102488, China; Email: liuyg0228@163.com 【Abstract 】 Objective To analyze the alkaloids in the different parts of Aconitum wulingense by HPLC-ESI-Trap-MS. Methods The Agilent XDB-C18(250 mm×4.6 mm, 5 μm) column with gradient elution of 0.1% solvent (A)-acetonitrile(B), at a flow rate of 1 ml/min was used. The column temperature was set at 30 ℃. The MS analysis was based on positive ions mode. Results In the roots, a total of 61 diterpenoid alkaloids were discovered, among which 46 were identified. In the stems, 38 alkaloids have been found, among which 33 alkaloids were identified and 27 were the same with the roots. In the leaves, 18 alkaloids have been detected and 8 were the same