实时荧光定量PCR检测转基因玉米Bt176测量不确定度分析.PDF

安徽农业大学学报, 2017, 44(1): 119-123 Journal of Anhui Agricultural University [DOI] 10.13610/ki.1672-352x023 网络出版时间：2017/2/8 10:25:00 [URL] /kcms/detail/34.1162.S1025.046.html 实时荧光定量 PCR 检测转基因玉米 Bt176 测量不确定度分析 * 熊 娟，黄启红 ，伍玲燕，蔡大川，张志军，黄璇莹，陈敏儿 ( 中国广州分析测试中心，广东省化学危害应急检测技术重点实验室，广州 510070) 摘 要：应用实时定量 PCR 方法测定样品中转基因玉米 Bt176 的含量，并从多个方面来分析测量的不确定度。 结果显示，A 类不确定度为 0.0003，B 类不确定度为 0.0007，合成不确定度为 0.001，有效自由度veff 为 272 ，包含 概率 P 为 95%，扩展不确定度 U=0.002 ，测量结果为（0.8±0.2）% 。不确定度评估结果表明，测量不确定度的主要 来源是微量液体转移量的偏差。 关键词：转基因玉米 Bt176 ；实时荧光定量PCR ；定量检测；不确定度评估 中图分类号：S513 文献标识码：A 文章编号：1672352X (2017)01011905 Uncertainty in detecting event-specific fragments of genetically modified maize BT176 by quantitative real time PCR XIONG Juan ，HUANG Qihong ，WU Lingyan ，CAI Dachuan，ZHANG Zhijun ，HUANG Xuanying ，CHEN Miner (Guangdong Provincial Key Laboratory of Emergency Test for Dangerous Chemicals, China National Analytical Center, Guangzhou 510070) Abstract: The real-time fluorescent quantitative PCR method was used to detect the event-specific fragments in a sample of genetically modified maize BT176 and the uncertainty of measurement derived from several as- pects was analyzed. The results showed that uncertainty of type A and type B was 0.0003 and 0.0007, respectively; the combined standard uncertainty was 0.001; the effective degree of freedom was 272; the coverage probability was 95%; the expanded uncertainty was 0.001, and the measured result was 0.8%±0.2%. This result indicated that the main source of uncertainty was the trace amount of liquid transfer, which should be paid more attention to in future detection. Key words: genetically modified maize Bt176; real-time fluorescent quantitative PCR method; quantitative detection; uncertainty evaluation 转基因食品(genetically