急性离心运动及针刺干预对骨骼肌细胞外基质的影响-体育学专业论文.docx

急性离心运动及针刺干预对骨骼肌细胞外基质的影响 急性离心运动及针刺干预对骨骼肌细胞外基质的影响 万方数据 万方数据 ABSTRACT Objectives The purpose of this project is to investigate skeletal muscle ultrastructure, the content changes of different type of collagens, the balance of MMPs/TIMPs in rat skeletal muscle extracellular matrix after acute eccentric exercise, analyse the relationship among MMP2, MMP9, TIMP1 and TIMP2, reveal the mechanism of acupuncture effect on exercise-induced skeletal muscle damage and remodeling extracellular matrix of skeletal muscle. Materials and Methods Eight-week-old Sprague-Dawley rats (n=96, body wt 220±6.9g), randomly assigned to 4 groups which named sedentary control(C), acupuncture control(CA), exercise group(E) and acupunctured exercise group(EA), were used in this study. The animals were adapted to the living room for 3 days and made to run on a motor-driven treadmill with -16° downhill tracks at a speed of 16m/min for total 90 min. Acupuncture control and acupunctured exercise rats were intervened after exercise. The animals were placed under anesthesia and killed by drawing blood from aorta abdominalis. The tissues were collected from vastus intermedius muscle of the experimental rats immediately, 24 hours, 48 hours, 72 hours, 120 hours after exercise. All the samples were stored in -80° fridge later. Transmission electron microscope(TEM) was used to observe the skeletal muscle ultrastructure, Scanning electron microscope(SEM) was used to analyse the morphological changes of collagen fibers, Western blotting and RT-PCR were adopted to detect gene and protein expression of type Ⅰ, Ⅲ, Ⅳcollagen, MMP2, MMP9, TIMP1 and TIMP2 in this research. Results Acute eccentric exercise could facilitate increasing of collagen 论文随机验证编号：BT510199501970 VI fibers, however acupuncture could inhibit it. SEM results revealed that collagen hyperplasia came up in skeletal muscle of E group rats, not EA rats, 120 hours post exercise. mRNA level of type Ⅰand Ⅲ collagen of E group rats were up regulated to ma