兔脉络膜上腔微电极阵列植入后视网膜电刺激阈值和OCT的研究观察.doc

 兔脉络膜上腔微电极阵列植入后视网膜电 刺激阈值和 OCT 的研究观察# 刘广峰，董方田，张华，于伟泓** 5 10 15 20 25 30 35 40 （北京协和医院眼科，北京 100005） 摘要：目的 研究兔眼脉络膜上腔多通道微电极阵列芯片植入术后，电刺激视网膜后在中枢 诱发的电诱发电位（EEP）的阈值和 OCT 表现。方法 将多通道刺激电极植入 9 只青紫蓝兔 左眼后极部的脉络膜上腔，将铂丝制成的参比电机置于玻璃体腔，硬膜外放置记录电极。刺 激电极采用双向刺激的方式发出电刺激，在中枢记录 EEP。应用检眼镜检查和 OCT 检查评价 多通道微电极阵列芯片植入和清除后对兔眼组织的安全性。结果 多通道微电极阵列芯片成 功植入 9 只实验兔眼的脉络膜上腔。当脉络膜上腔刺激电极发出电刺激后，可以在硬脑膜外 2 OCT 检查显示相应的脉络膜组织与邻近组织间连接紧密。 结论 置于脉络膜上腔的电极发 出的电刺激可以有效地诱发出 EEP，且其阈值较低；脉络膜上腔是较理想的视网膜假体放置 部位。 关键词：眼科学；微电极阵列；脉络膜上腔电极；电诱发电位；阈值；OCT 中图分类号：R773.404 OCT change and threshold of electrical evoked potentials stimulated by a suprachoroidal multichannel electrode array in rabbit eyes Liu Guangfeng, Dong Fangtian, Zhang Hua, Yu Weihong (Department of Ophthalmology, Peking Union Medical College Hospital, Beijing 100005) Abstract: Objective The aim of this study is to investigate the OCT change and threshold electrical charge density of the retina in rabbits for the generation of electrical evoked potentials (EEP) by delivering electrical stimulation with a microelectrode array (MEA) implanted into the suprachoroidal space. Methods A flat electrode array was developed and applied in this study. The left eyes of 9 chinchilla rabbits were selected as experimental eyes. After performing a scleral incision at 6 mm from the limbus, the array was inserted into the suprachoroidal space in the posterior portion of the eye. A platinum wire was implanted into the vitreous space as a reference electrode. For electrical stimulation, a biphasic pulse was used. When the electrode was stimulated, the EEP was recorded. The safety of this device to eye tissue was evaluated by ophthalmoscope, and OCT examination. Results The MEA was successfully implanted into the retina and suprachoroidal space. When the electrical stimulation from the superchoroidal space was applied, the EEP could be recorded with an epidural electrode, and the threshold was 107.14 ± 17.14 nC (68.20 ±10.91 μC/cm2). OCT revealed that the device attached to choriod and adjacen