离体筛选茄子抗羟脯氨酸突变体的初步研究-蔬菜学专业论文.docxVIP

离体筛选茄子抗羟脯氨酸突变体的初步研究-蔬菜学专业论文.docx

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AbstractEggplant(Sotanum Abstract Eggplant(Sotanum melongena L.)is a kind of popular vegetable with abundant nutrition,special flavor as well as peculiar medical value.In recent years,with the increase of eggplant consumption and the enlargement of its area of protected cultivation, the demand of the special variety for protected cultivation is increasing.But nowadays the varieties of eggplant for this purpose call’t tolerate cold and low-light stress very well,so it is quite necessary to cultivate this kind ofvarieties. Since the hybrid varieties of eggplant ale popularized continuously,many regional varieties have been lost and eggplant’s genetic background has been narrowed.So far,as the cold·tolerant materials haven’t been found in cultivars,it is very difficult to obtain cold—tolerant varieties by conventional means.Zhlough gene transferring and somatic hybridization,cold—tolerant varieties have been obtained and the exploitation of somaclonal variation of some crops also succeeds.The variation is quite common among regenerated plants after tissue culture,and the frequency of mutation is very high.It can occur both at chromosomal and molecular levels. And many disease—resistant and adversity-tolerant varieties have been developed through in vitro selection with stress. With the condition of our laboratory,the in vitro culturing procedure of eggplant had been well established and through in vitro selection cold—tolerant calli and their regenerated plants had been obtained in the research.The main results were as follows: 1.Calli from hypocotyl and cotyledon explants of eggplant were obtained on MS culture medium supplemented with NAA and BA of different concentrations,but there was distinctive difference among genotypes.The combination of BA and NAA had great influence on callus induction of eggplant.One of these combinations,2.0 mg/L BA and 0.2 mg/L NAA,induced a type of compact callus in color varied from light yellow to light green,which was beneficial to dif

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