莱氏野村菌钠钙交换体和钙离子通道抑制子基因的克隆与功能分析-生物学专业论文.docxVIP

重庆大学硕士学位论文英文摘要 重庆大学硕士学位论文 英文摘要 PAGE PAGE IV ABSTRACT Nomuraea rileyi as an insect pathogenic fungi, can cause insects epidemic in the fields thus received extensive attention of people. But as the main effective components of insect fungus - conidia, the sporulation of N. rileyi required culture conditions are harsh, which limits its industrial production and large-scale application. The research group of Zhongkang Wang in Chongqing University used the induction method of plant pathogenic fungi MS successfully induced microsclerotium(MS) of N. rileyi. MS as a special infect structure, with the advantages of industrial production, high temperature resistant, resistant storage, strong resistance and others can replace traditional conidia. Then they used the method of comparative transcriptome to explore the formation mechanism of MS. At the same time in the optimization of induction medium found that adding different concentrations of Ca2+ MS yielded remarkably different. So this paper selected genes related to Na+-Ca2+ Exchanger (NCX) and Calcium Channel Inhibitor (CCI) from the comparative transcriptome of N. rileyi to study the relationship between calcium and MS of N. rileyi. The main results are as follows: ① By adding Ca2+ in the induction medium found that in AM-Ca2+ free medium the size of mycelium pellet was heterogeneity and decreased by 34.4%. And the hyphae twined loosely, there also are a lot of hyphae, puce pigment accumulation decreased obviously. The biomass statistics found that adding Ca2+ or not biomass was not significantly change, so it didnt affect the growth of N. rileyi; ② According to the NCX and CCI EST sequence of the comparative transcriptome, obtained the NCX and CCI full-length cDNA and genomic DNA sequences by narmal PCR. open reading frame (ORF) of NCX and CCI are 3006bp and 405bp, encoding 1001 and 134 amino acids respectively, Using the bioinformatics software and web site analysis the NCX and CCI found: the protein mo